Department of Respiratory and Critical Care Medicine of Zhongshan Hospital, Shanghai Institute of Respiratory Disease, Fudan University, Shanghai, People's Republic of China.
Geriatric Department of Zhongshan Hospital, Shanghai Institute of Respiratory Disease, Fudan University, Shanghai, People's Republic of China.
Int J Chron Obstruct Pulmon Dis. 2023 Apr 27;18:705-717. doi: 10.2147/COPD.S400999. eCollection 2023.
The progression of an abnormal inflammatory response plays a crucial role in the lung function decline of chronic obstructive pulmonary disease (COPD) patients. Compared to serum biomarkers, inflammatory biomarkers in induced sputum would be a more reliable reflection of inflammatory processes in the airways.
A total of 102 COPD participants were divided into a mild-to-moderate group (FEV1%pred≥ 50%, n=57) and a severe-to-very-severe group (FEV1%pred<50%, n=45). We measured a series of inflammatory biomarkers in induced sputum and analyzed their association with lung function and SGRQ in COPD patients. To evaluate the relationship between inflammatory biomarkers and the inflammatory phenotype, we also analyzed the correlation between biomarkers and airway eosinophilic phenotype.
We found increased mRNA levels of MMP9, LTB4R, and A1AR and decreased levels of CC16 mRNA in induced sputum in the severe-to-very-severe group. After adjustment for age, sex and other biomarkers, CC16 mRNA expression was positively associated with FEV1%pred (r=0.516, p=0.004) and negatively correlated with SGRQ scores (r=-0.3538, p=0.043). As previously known, decreased CC16 was related to the migration and aggregation of eosinophils in airway. It was also found that CC16 had a moderate negative correlation with the eosinophilic inflammation in airway (r=-0.363, p=0.045) in our COPD patients.
Low CC16 mRNA expression levels in induced sputum were associated with low FEV1%pred and a high SGRQ score in COPD patients. Sputum CC16 as a potential biomarker for predicting COPD severity in clinical practice might attribute to the involvement of CC16 in airway eosinophilic inflammation.
异常炎症反应的进展在慢性阻塞性肺疾病（COPD）患者的肺功能下降中起着至关重要的作用。与血清生物标志物相比，诱导痰中的炎症生物标志物更能可靠地反映气道中的炎症过程。
共纳入 102 例 COPD 患者，分为轻度至中度组（FEV1%pred≥50%，n=57）和重度至极重度组（FEV1%pred<50%，n=45）。我们测量了诱导痰中的一系列炎症生物标志物，并分析了它们与 COPD 患者肺功能和 SGRQ 的关系。为了评估炎症生物标志物与炎症表型之间的关系，我们还分析了生物标志物与气道嗜酸性粒细胞表型之间的相关性。
我们发现重度至极重度组诱导痰中 MMP9、LTB4R 和 A1AR 的 mRNA 水平升高，CC16mRNA 水平降低。在校正年龄、性别和其他生物标志物后，CC16mRNA 表达与 FEV1%pred 呈正相关（r=0.516，p=0.004），与 SGRQ 评分呈负相关（r=-0.3538，p=0.043）。如前所述，CC16 减少与气道中嗜酸性粒细胞的迁移和聚集有关。我们还发现，CC16 与 COPD 患者气道中的嗜酸性粒细胞炎症呈中度负相关（r=-0.363，p=0.045）。
COPD 患者诱导痰中 CC16mRNA 表达水平低与 FEV1%pred 降低和 SGRQ 评分高有关。痰 CC16 作为预测 COPD 严重程度的潜在生物标志物，可能与 CC16 参与气道嗜酸性粒细胞炎症有关。
