Ye Jun, Yao Jian-Ping, Wang Xu, Zheng Minying, Li Peng, He Chengwei, Wan Jian-Bo, Yao Xiaoli, Su Huanxing
Department of Dermatology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zheijiang 310016, P.R. China.
Department of Cardiac Surgery II, The First Affiliated Hospital Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.
Mol Med Rep. 2016 Apr;13(4):3083-91. doi: 10.3892/mmr.2016.4914. Epub 2016 Feb 19.
Ginsenosides exhibit various neuroprotective effects against oxidative stress. However, which ginsenoside provides optimal effects for the treatment of neurological disorders as a potent antioxidant remains to be elucidated. Therefore, the present study investigated and compared the neuroprotective effects of the Rb1, Rd, Rg1 and Re ginsenosides on neural progenitor cells (NPCs) following tert-Butylhydroperoxide (t-BHP)-induced oxidative injury. Primary rat embryonic cortical NPCs were prepared from E14.5 embryos of Sprague-Dawley rats. The oxidative injury model was established with t‑BHP. A lactate dehydrogenase assay and terminal deoxynucleotidyl transferase dUTP nick‑end labeling staining were used to measure the viability of the NPCs pre‑treated with ginsenosides under oxidative stress. Reverse transcription‑quantitative polymerase chain reaction analysis was used to determine the activation of intracellular signaling pathways triggered by the pretreatment of ginsenosides. Among the four ginsenosides, only Rb1 attenuated t‑BHP toxicity in the NPCs, and the nuclear factor (erythroizd‑derived 2)‑like 2/heme oxygenase‑1 pathway was found to be key in the intracellular defense against oxidative stress. The present study demonstrated the anti-oxidative effects of ginsenoside Rb1 on NPCs, and suggested that Rb1 may offer potential as a potent antioxidant for the treatment of neurological disorders.
人参皂苷对氧化应激具有多种神经保护作用。然而，作为一种有效的抗氧化剂，哪种人参皂苷对神经系统疾病的治疗效果最佳仍有待阐明。因此，本研究调查并比较了人参皂苷Rb1、Rd、Rg1和Re对叔丁基过氧化氢（t-BHP）诱导的神经祖细胞（NPCs）氧化损伤后的神经保护作用。原代大鼠胚胎皮质NPCs取自Sprague-Dawley大鼠E14.5胚胎。用t-BHP建立氧化损伤模型。采用乳酸脱氢酶测定法和末端脱氧核苷酸转移酶dUTP缺口末端标记染色法检测氧化应激下人皂苷预处理后NPCs的活力。采用逆转录-定量聚合酶链反应分析来确定人参皂苷预处理触发的细胞内信号通路的激活情况。在这四种人参皂苷中，只有Rb1能减轻NPCs中的t-BHP毒性，并且发现核因子（红系衍生2）样2/血红素加氧酶-1途径是细胞内抗氧化应激防御的关键。本研究证明了人参皂苷Rb1对NPCs的抗氧化作用，并表明Rb1作为一种有效的抗氧化剂在治疗神经系统疾病方面可能具有潜力。
