Sin Celine, Chiarugi Davide, Valleriani Angelo
Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany.
Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany
Nucleic Acids Res. 2016 Apr 7;44(6):2528-37. doi: 10.1093/nar/gkw137. Epub 2016 Mar 1.
Premature ribosome drop-off is one of the major errors in translation of mRNA by ribosomes. However, repeated analyses of Ribo-seq data failed to quantify its strength inE. coli Relying on a novel highly sensitive data analysis method we show that a significant rate of ribosome drop-off is measurable and can be quantified also when cells are cultured under non-stressing conditions. Moreover, we find that the drop-off rate is highly variable, depending on multiple factors. In particular, under environmental stress such as amino acid starvation or ethanol intoxication, the drop-off rate markedly increases.
核糖体过早解离是核糖体翻译信使核糖核酸（mRNA）过程中的主要错误之一。然而，对核糖体图谱测序（Ribo-seq）数据的反复分析未能量化其在大肠杆菌中的发生强度。依靠一种新型的高灵敏度数据分析方法，我们发现，即使在非应激条件下培养细胞时，核糖体解离的显著发生率也是可测量且可量化的。此外，我们发现解离率高度可变，取决于多种因素。特别是，在诸如氨基酸饥饿或乙醇中毒等环境应激条件下，解离率会显著增加。
