Awad Sherine, Valleriani Angelo, Chiarugi Davide
Genomics and Bioinformatics Core Facility, Institute of Metabolic Science, University of Cambridge, Cambridge, CB2 0QQ, UK.
Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14424 Potsdam, Germany.
NAR Genom Bioinform. 2024 Apr 18;6(2):lqae036. doi: 10.1093/nargab/lqae036. eCollection 2024 Jun.
Ribosomes are the molecular machinery that catalyse all the fundamental steps involved in the translation of mRNAs into proteins. Given the complexity of this process, the efficiency of protein synthesis depends on a large number of factors among which ribosome drop-off (i.e. the premature detachment of the ribosome from the mRNA template) plays an important role. However, an quantification of the extent to which ribosome drop-off occurs is not trivial due to difficulties in obtaining the needed experimental evidence. In this work we focus on the study of ribosome drop-off in by using 'Ribofilio', a novel software tool that relies on a high sensitive strategy to estimate the ribosome drop-off rate from ribosome profiling data. Our results show that ribosome drop-off events occur at a significant rate also when is cultured in standard conditions. In this context, we also identified a correlation between the ribosome drop-off rate and the genes length: the longer the gene, the lower the drop-off rate.
核糖体是催化信使核糖核酸（mRNA）翻译成蛋白质所涉及的所有基本步骤的分子机器。鉴于这一过程的复杂性，蛋白质合成的效率取决于大量因素，其中核糖体脱落（即核糖体从mRNA模板上过早脱离）起着重要作用。然而，由于难以获得所需的实验证据，对核糖体脱落发生程度的量化并非易事。在这项工作中，我们通过使用“Ribofilio”专注于研究核糖体脱落，“Ribofilio”是一种新颖的软件工具，它依靠一种高灵敏度策略从核糖体谱数据估计核糖体脱落率。我们的结果表明，即使在标准条件下培养时，核糖体脱落事件也以显著的速率发生。在此背景下，我们还确定了核糖体脱落率与基因长度之间的相关性：基因越长，脱落率越低。
