Andrews Megan Y, Santelli Cara M, Duckworth Owen W
Department of Soil Science, North Carolina State University, Raleigh, NC 27695-7619, USA.
University of Minnesota, Department of Earth Sciences, Minneapolis, MN 55455-0231, USA.
Data Brief. 2016 Feb 3;6:890-8. doi: 10.1016/j.dib.2016.01.054. eCollection 2016 Mar.
This article presents visual image data and detailed methodology for the use of a new method for quantifying the exudation of siderophores during fungal growth. The data include images showing time series for calibration, fungal exudation, and negative controls, as well as replication accuracy information. In addition, we provide detailed protocols for making CAS assay layer plates, the digital analysis protocol for determining area of color change, and discuss growth media that do and do not work with the layer plate method. The results of these data, their interpretation, and further discussion can be found in Andrews et al., 2016 [1].
本文展示了视觉图像数据以及使用一种新方法量化真菌生长过程中铁载体渗出量的详细方法。数据包括用于校准、真菌渗出和阴性对照的时间序列图像，以及复制准确性信息。此外，我们提供了制作CAS测定层板的详细方案、用于确定颜色变化区域的数字分析方案，并讨论了适用于和不适用于层板法的生长培养基。这些数据的结果、解释及进一步讨论可在Andrews等人2016年的文献[1]中找到。
