Caplan Y H, Levine B
University of Maryland, Department of Pathology, Baltimore 21201.
J Anal Toxicol. 1989 Sep-Oct;13(5):289-92. doi: 10.1093/jat/13.5.289.
This study evaluated the capability of the Abbott ADx assays to test for phencyclidine (PCP) and barbiturates in urine specimens by comparison to TDx, EMIT, and gas chromatographic/mass spectrometric (GC/MS) methods. Within-run and between-run precision were determined using three control samples (35, 100, and 250 ng/mL PCP; 0.4, 0.6, and 1.0 mg/L secobarbital) tested over a two-week period. Controls were analyzed in both single assay (batch) and multiple assay (combination) modes. Within-run coefficients of variation (CVs) for the three PCP controls were 2.4, 2.6, and 2.2%, respectively; the between-run CVs were 4.0, 3.5, and 2.8%, respectively. The within-run CVs for the three barbiturate controls were 3.0, 2.7, and 2.7%, respectively; the between-run CVs were 5.6, 4.7, and 4.5%, respectively. Fifty specimens not containing PCP or barbiturates, 50 specimens containing PCP, and 50 specimens containing barbiturates were tested by ADx, TDx, EMIT, and GC/MS. The methods compared favorably and no false positive or false negative results were obtained by any immunoassay method for any assay when compared to GC/MS.
本研究通过与TDx、EMIT和气相色谱/质谱（GC/MS）方法相比较，评估了雅培ADx检测法对尿液标本中苯环己哌啶（PCP）和巴比妥类药物的检测能力。使用三个对照样品（35、100和250 ng/mL的PCP；0.4、0.6和1.0 mg/L的司可巴比妥）在两周时间内进行检测，以确定批内和批间精密度。对照样品在单次检测（批次）和多次检测（组合）模式下进行分析。三个PCP对照样品的批内变异系数（CVs）分别为2.4%、2.6%和2.2%；批间CVs分别为4.0%、3.5%和2.8%。三个巴比妥类药物对照样品的批内CVs分别为3.0%、2.7%和2.7%；批间CVs分别为5.6%、4.7%和4.5%。通过ADx、TDx、EMIT和GC/MS对50份不含PCP或巴比妥类药物的标本、50份含PCP的标本和50份含巴比妥类药物的标本进行了检测。这些方法相比效果良好，与GC/MS相比，任何免疫检测方法在任何检测中均未出现假阳性或假阴性结果。
