Fraser A D, Bryan W
Toxicology Laboratory, Victoria General Hospital, Halifax, Nova Scotia, Canada.
J Anal Toxicol. 1991 Mar-Apr;15(2):63-5. doi: 10.1093/jat/15.2.63.
This study evaluated the Abbott ADx and TDx serum benzodiazepine assays for the analysis of alprazolam in serum. These systems were compared to the EMIT tox serum benzodiazepine assay and a gas chromatographic (GC) method. Six-point calibration curves (nordiazepam concentrations from 50-1,000 ng/mL) were stable on both instruments for at least 14 days. The precision of these assays was determined using three control serums containing 75,300, and 700 ng/mL of nordiazepam. On the ADx, within-run coefficients of variation (CV) for the three controls were 3.9, 3.1, and 2.8%, respectively. Between-run CVs were 2.8, 1.4, and 1.2% on the ADx. For the TDx, within-run CVs were 1.9, 1.4, and 2.6, and between-run CVs were 4.1, 1.3, and 1.9% for the three controls. Percent cross-reactivity of serum alprazolam calibrators (25-100 ng/mL) ranged from 77-88% on the ADx and 60-79% on the TDx. 99 serum specimens containing alprazolam were analyzed with the ADx, TDx, EMIT, and by gas chromatography-electron capture detection (sensitivity of 5 ng/mL). Overall, 48 of 99 specimens on the TDx, 43 of 99 on the ADx (both with a cutoff of 12 ng/mL), and 0 of 99 by EMIT (cutoff of 300 ng/mL) were positive. Of the specimens reading negative on the Abbott systems, 43 of 51 TDx results and 44 of 56 ADx results were obtained in specimens containing less than 25 ng/mL of alprazolam. For specimens with more than 25 ng/mL of alprazolam in serum (by GC), 8 specimens were negative on the TDx and 12 on the ADx.
本研究评估了雅培ADx和TDx血清苯二氮䓬检测法用于分析血清中阿普唑仑的情况。将这些检测系统与EMIT tox血清苯二氮䓬检测法及气相色谱（GC）法进行了比较。六点校准曲线（去甲西泮浓度为50 - 1000 ng/mL）在两台仪器上至少14天保持稳定。使用三种分别含有75、300和700 ng/mL去甲西泮的对照血清来测定这些检测法的精密度。在ADx上，三种对照的批内变异系数（CV）分别为3.9%、3.1%和2.8%。ADx的批间CV分别为2.8%、1.4%和1.2%。对于TDx，三种对照的批内CV分别为1.9%、1.4%和2.6%，批间CV分别为4.1%、1.3%和1.9%。血清阿普唑仑校准品（25 - 100 ng/mL）的交叉反应率在ADx上为77 - 88%，在TDx上为60 - 79%。用ADx、TDx、EMIT及气相色谱 - 电子捕获检测法（灵敏度为5 ng/mL）分析了99份含阿普唑仑的血清标本。总体而言，TDx上99份标本中有48份阳性，ADx上99份中有43份阳性（两者临界值均为12 ng/mL），EMIT上99份中0份阳性（临界值为300 ng/mL）。在雅培检测系统上读数为阴性的标本中，51份TDx结果中有43份以及56份ADx结果中有44份是在阿普唑仑含量低于25 ng/mL的标本中获得的。对于血清中阿普唑仑含量超过25 ng/mL的标本（通过GC测定），TDx上有8份结果为阴性，ADx上有12份结果为阴性。
