Setou Mitsutoshi, Shrivas Kamlesh, Sroyraya Morakot, Yang Hyunjeong, Sugiura Yuki, Moribe Junji, Kondo Akira, Tsutsumi Koji, Kimura Yoshishige, Kurabe Nobuya, Hayasaka Takahiro, Goto-Inoue Naoko, Zaima Nobuhiro, Ikegami Koji, Sobhon Prasert, Konishi Yoshiyuki
Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
Med Mol Morphol. 2010 Mar;43(1):1-5. doi: 10.1007/s00795-009-0489-0. Epub 2010 Mar 26.
We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on some of the recent developments in clinical applications to show how mass microscopy can be employed to assess medical molecular morphology.
我们开发了一种质谱显微镜技术，即一种结合了高分辨率基质辅助激光解吸/电离成像质谱（MALDI-IMS）的显微镜，它是一种强大的工具，可用于研究生物分子的空间分布，而无需对生物组织切片进行任何耗时的提取、纯化和分离程序。质谱显微镜在单次测量中就能提供数百种生物分子分布的清晰图像，还有助于了解生物系统的细胞概况。该技术的样品制备、空间分辨率和速度都是影响质谱显微镜中生物分子鉴定的重要步骤。在本次获奖讲座综述中，我们重点介绍临床应用方面的一些最新进展，以展示如何利用质谱显微镜来评估医学分子形态。
