Matsushita Y, Kitakawa M, Isono K
Department of Biology, Faculty of Science, Kobe University, Japan.
Mol Gen Genet. 1989 Oct;219(1-2):119-24. doi: 10.1007/BF00261166.
Two mitochondrial ribosomal proteins of yeast (Saccharomyces cerevisiae) were purified and their N-terminal amino acid sequences determined. The sequence data were used for the synthesis of oligonucleotide probes to clone the corresponding genes. Thus, the genes for two proteins, termed YMR-31 and YMR-44, were cloned and their nucleotide sequences determined. From the nucleotide sequence data, the coding region of the gene for protein YMR-31 was found to be composed of 369 nucleotide pairs. Comparison of the amino acid sequence of protein YMR-31 and the one deduced from the nucleotide sequence of its gene suggests that it contains an octapeptide leader sequence. The calculated molecular weight of protein YMR-31 without the leader sequence is 12,792 dalton. The gene for protein YMR-44 was found to contain a 147 bp intron which contains two sequences conserved among yeast introns. The length of the two exons flanking the intron totals 294 nucleotide pairs which can encode a protein with a calculated molecular weight of 11,476 dalton. The gene for protein YMR-31 is located on chromosome VI, while the gene for protein YMR-44 is located on either chromosome XIII or XVI.
纯化了酵母（酿酒酵母）的两种线粒体核糖体蛋白，并测定了它们的N端氨基酸序列。序列数据用于合成寡核苷酸探针以克隆相应基因。因此，克隆了两种名为YMR - 31和YMR - 44的蛋白质的基因，并测定了它们的核苷酸序列。从核苷酸序列数据中发现，蛋白质YMR - 31的基因编码区由369个核苷酸对组成。蛋白质YMR - 31的氨基酸序列与其基因核苷酸序列推导的氨基酸序列的比较表明，它含有一个八肽前导序列。不含前导序列的蛋白质YMR - 31的计算分子量为12,792道尔顿。发现蛋白质YMR - 44的基因含有一个147 bp的内含子，该内含子包含酵母内含子中保守的两个序列。内含子两侧的两个外显子的长度总计294个核苷酸对，可编码一种计算分子量为11,476道尔顿的蛋白质。蛋白质YMR - 31的基因位于第六条染色体上，而蛋白质YMR - 44的基因位于第十三条或第十六条染色体上。
