Glazer V M, Glazunov A V, Tevzadze G G, Koltovaia N A
Mol Gen Mikrobiol Virusol. 1989 Sep(9):14-20.
The repair of a double strand gap in plasmid DNA in radiosensitive mutants of Saccharomyces cerevisiae has been studied. The proportion of repair events resulting in the complete doublestrand gap recovery of the plasmid DNA has been found to be close to 100% in Rad+ cells. The mutation rad55 did not interfere in the doublestrand gap repair efficiency and accuracy. The mutant rad57 is capable of the effective doublestrand gap repair without restoration of the DNA sequence deleted by the gap. The mutation rad53 substantially inhibited the efficiency of the doublestrand gap repair but did not influence the accuracy of the repair. Plasmid DNA doublestranded gap repair is completely blocked by mutations rad50 and rad54.
对酿酒酵母辐射敏感突变体中质粒DNA双链缺口的修复进行了研究。已发现，在Rad+细胞中，导致质粒DNA双链缺口完全恢复的修复事件比例接近100%。rad55突变不影响双链缺口修复效率和准确性。rad57突变体能够有效地进行双链缺口修复，而不会恢复被缺口删除的DNA序列。rad53突变显著抑制了双链缺口修复效率，但不影响修复的准确性。rad50和rad54突变完全阻断了质粒DNA双链缺口的修复。
