Pianigiani E, Tognetti L, Ierardi F, Mariotti G, Rubegni P, Cevenini G, Perotti R, Fimiani M
Dermatology Unit and Skin Bank, Department of Clinical Medicine and Immunological Science, University of Siena, "Le Scotte" Hospital - AOUS, V.le Bracci, 53100, Siena, Italy.
Department of Medical Biotechnologies, University of Siena, Siena, Italy.
Cell Tissue Bank. 2016 Jun;17(2):241-53. doi: 10.1007/s10561-016-9550-0. Epub 2016 Mar 3.
Skin allografts from cadaver donors are an important resource for treating extensive burns, slow-healing wounds and chronic ulcers. A high level of cell viability of cryopreserved allografts is often required, especially in burn surgery, in Italy. Thus, we aimed to determine which conditions enable procurement of highly viable skin in our Regional Skin Bank of Siena. For this purpose, we assessed cell viability of cryopreserved skin allografts procured between 2011 and 2013 from 127 consecutive skin donors, before and after freezing (at day 15, 180, and 365). For each skin donor, we collected data concerning clinical history (age, sex, smoking, phototype, dyslipidemia, diabetes, cause of death), donation process (multi-tissue or multi-organ) and timing of skin procurement (assessment of intervals such as death-harvesting, harvesting-banking, death-banking). All these variables were analysed in the whole case study (127 donors) and in different groups (e.g. multi-organ donors, non refrigerated multi-tissue donors, refrigerated multi-tissue donors) for correlations with cell viability. Our results indicated that cryopreserved skin allografts with higher cell viability were obtained from female, non smoker, heartbeating donors died of cerebral haemorrhage, and were harvested within 2 h of aortic clamping and banked within 12 h of harvesting (13-14 h from clamping). Age, cause of death and dyslipidaemia or diabetes did not appear to influence cell viability. To maintain acceptable cell viability, our skin bank needs to reduce the time interval between harvesting and banking, especially for refrigerated donors.
来自尸体供体的皮肤异体移植物是治疗大面积烧伤、愈合缓慢的伤口和慢性溃疡的重要资源。在意大利，尤其是在烧伤手术中，通常需要冷冻保存的异体移植物具有较高的细胞活力。因此，我们旨在确定哪些条件能够使我们锡耶纳地区皮肤库获取高活力的皮肤。为此，我们评估了2011年至2013年间从127名连续皮肤供体获取的冷冻保存皮肤异体移植物在冷冻前后（第15天、180天和365天）的细胞活力。对于每位皮肤供体，我们收集了有关临床病史（年龄、性别、吸烟情况、肤色类型、血脂异常、糖尿病、死因）、捐赠过程（多组织或多器官）以及皮肤获取时间（评估死亡至获取、获取至入库、死亡至入库等间隔时间）的数据。在整个案例研究（127名供体）以及不同组（如多器官供体、非冷藏多组织供体、冷藏多组织供体）中分析所有这些变量与细胞活力的相关性。我们的结果表明，细胞活力较高的冷冻保存皮肤异体移植物来自女性、不吸烟、因脑出血死亡的心跳供体，并且在主动脉钳夹后2小时内获取并在获取后12小时内入库（从钳夹开始13 - 14小时）。年龄、死因以及血脂异常或糖尿病似乎并未影响细胞活力。为保持可接受的细胞活力，我们的皮肤库需要缩短获取和入库之间的时间间隔，尤其是对于冷藏供体。
