Pianigiani E, Ierardi F, Cuciti C, Brignali S, Oggioni M, Fimiani M
Siena Skin Bank, Department Dermatology, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy.
Burns. 2010 May;36(3):347-51. doi: 10.1016/j.burns.2009.04.020. Epub 2009 Jul 17.
The Siena Skin Bank, established in 2000, processes skin from more than 130 cadaveric donors per year (about 400,000 cm(2)) and distributes it for transplants to treat burns and other types of skin loss. More than 1,500,000 cm(2) of homologous skin has been transplanted to date. At the Siena Skin Bank we conducted a retrospective study of our data to assess microbial contamination of skin specimens from 723 donors banked in the period 2000-2007. Our aim was to determine factors deleterious for skin quality, to optimize skin banking procedures and to reduce corrective actions. The factors analyzed were: type of donor (multi-organ, multi-tissue, live or cadaver), cause of death, time elapsing between death and procurement, different procurement centres and operator experience. Of the 723 donors considered, 26.55% (192/723) were positive for microbes, 22.68% (164) for bacteria and 5.39% (39) for mycetes. Of these 192 positives, 82 (42.70%) required corrective actions. The data obtained showed that the only variables significantly affecting microbial contamination of tissue were type of donor (live or cadaver) and type of processing (cryo- or glycerol preservation).
锡耶纳皮肤库成立于2000年，每年处理来自130多名尸体捐赠者的皮肤（约400,000平方厘米），并将其分发用于移植，以治疗烧伤和其他类型的皮肤缺损。迄今为止，已移植了超过1,500,000平方厘米的同种异体皮肤。在锡耶纳皮肤库，我们对数据进行了回顾性研究，以评估2000年至2007年期间储存的723名捐赠者皮肤标本的微生物污染情况。我们的目的是确定对皮肤质量有害的因素，优化皮肤储存程序并减少纠正措施。分析的因素包括：捐赠者类型（多器官、多组织、活体或尸体）、死亡原因、死亡与获取之间的时间间隔、不同的获取中心和操作人员经验。在723名被考虑的捐赠者中，26.55%（192/723）的微生物检测呈阳性，22.68%（164）为细菌阳性，5.39%（39）为真菌阳性。在这192例阳性中，82例（42.70%）需要采取纠正措施。获得的数据表明，对组织微生物污染有显著影响的唯一变量是捐赠者类型（活体或尸体）和处理类型（冷冻或甘油保存）。
