Ko S D, Page R C, Narayanan A S
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3429-32. doi: 10.1073/pnas.74.8.3429.
The effects of prostaglandin E(2) (PGE(2)) upon the synthesis of protein and DNA, and membrane transport of proline and thymidine, by human diploid fibroblasts were studied. At a concentration range of 1-10 muM, PGE(2) inhibited protein synthesis and membrane transport by 45-50%. Serum-activated DNA synthesis and thymidine transport were also inhibited by approximately 50% in cells made quiescent and synchronous by serum deprivation. To determine whether prostaglandin inhibits some of the cells completely or all of the cells partially, radioautographic and cell-counting experiments were done. In cultures pulse-labeled with [(3)H]thymidine 12-33 hr after serum activation, prostaglandin exposure reduced the number of labeled nuclei by 42%. Sixty-five hours after serum activation, the total cell numbers present in the PGE(2)-exposed cultures were reduced by 25%. Furthermore, in the fibroblast cultures derived from cells previously maintained in 10 muM PGE(2) for 14 days, PGE(2) had no effect on DNA synthesis, indicating that the PGE(2)-sensitive cells had disappeared from the cultures. Thus, PGE(2) appears to inhibit growth and synthesis of a subpopulation of cells while not affecting the remaining insensitive cells. Prostaglandins may play an important role in connective-tissue differentiation and in some pathologic alterations by regulating fibroblast subpopulations.
研究了前列腺素E(2)（PGE(2)）对人二倍体成纤维细胞蛋白质和DNA合成以及脯氨酸和胸苷膜转运的影响。在1 - 10μM的浓度范围内，PGE(2)抑制蛋白质合成和膜转运45 - 50%。在通过血清剥夺使细胞静止并同步化的细胞中，血清激活的DNA合成和胸苷转运也被抑制了约50%。为了确定前列腺素是完全抑制部分细胞还是部分抑制所有细胞，进行了放射自显影和细胞计数实验。在血清激活后12 - 33小时用[(3)H]胸苷脉冲标记的培养物中，暴露于前列腺素后标记细胞核的数量减少了42%。血清激活65小时后，暴露于PGE(2)的培养物中的总细胞数减少了25%。此外，在先前在10μM PGE(2)中维持14天的细胞来源的成纤维细胞培养物中，PGE(2)对DNA合成没有影响，这表明对PGE(2)敏感的细胞已从培养物中消失。因此，PGE(2)似乎抑制了一部分细胞的生长和合成，而不影响其余不敏感的细胞。前列腺素可能通过调节成纤维细胞亚群在结缔组织分化和某些病理改变中起重要作用。
