外源蛋白原性氨基酸可诱导水稻产生系统抗性。

Exogenous proteinogenic amino acids induce systemic resistance in rice.

作者信息

Kadotani Naoki, Akagi Aya, Takatsuji Hiroshi, Miwa Tetsuya, Igarashi Daisuke

机构信息

Institute for Innovation, Ajinomoto Co., Inc, 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki, 210-8681, Japan.

Plant Disease Resistance Research Unit, Division of Plant Science, National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, 305-8602, Japan.

出版信息

BMC Plant Biol. 2016 Mar 3;16:60. doi: 10.1186/s12870-016-0748-x.

BACKGROUND

Plant immune responses can be induced by endogenous and exogenous signaling molecules. Recently, amino acids and their metabolites have been reported to affect the plant immune system. However, how amino acids act in plant defense responses has yet to be clarified. Here, we report that treatment of rice roots with amino acids such as glutamate (Glu) induced systemic disease resistance against rice blast in leaves.

RESULTS

Treatment of roots with Glu activated the transcription of a large variety of defense-related genes both in roots and leaves. In leaves, salicylic acid (SA)-responsive genes, rather than jasmonic acid (JA) or ethylene (ET)-responsive genes, were induced by this treatment. The Glu-induced blast resistance was partially impaired in rice plants deficient in SA signaling such as NahG plants expressing an SA hydroxylase, WRKY45-knockdown, and OsNPR1-knockdown plants. The JA-deficient mutant cpm2 exhibited full Glu-induced blast resistance.

CONCLUSIONS

Our results indicate that the amino acid-induced blast resistance partly depends on the SA pathway but an unknown SA-independent signaling pathway is also involved.

背景

植物免疫反应可由内源性和外源性信号分子诱导。最近，有报道称氨基酸及其代谢产物会影响植物免疫系统。然而，氨基酸在植物防御反应中的作用机制尚待阐明。在此，我们报道用谷氨酸（Glu）等氨基酸处理水稻根部可诱导叶片对稻瘟病产生系统性抗病性。

结果

用Glu处理根部可激活根和叶中多种防御相关基因的转录。在叶片中，这种处理诱导的是水杨酸（SA）响应基因，而非茉莉酸（JA）或乙烯（ET）响应基因。在缺乏SA信号传导的水稻植株中，如表达SA羟化酶的NahG植株、WRKY45基因敲低植株和OsNPR1基因敲低植株，Glu诱导的稻瘟病抗性部分受损。JA缺陷型突变体cpm2表现出完全的Glu诱导的稻瘟病抗性。