Zhang Jian-Ping, Xu Xi-Ke, Ye Ji, Yang Yong-Xun, Gao Shuang, Li Hui-Liang, Zhang Wei-Dong
School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
Fitoterapia. 2016 Apr;110:72-6. doi: 10.1016/j.fitote.2016.02.013. Epub 2016 Mar 3.
Three new sesquiterpene lactone dimers (SLDs), carpedilactones E-G (1-3), together with two known monomeric units, ivalin (4) and alantolactone (5), were isolated from the acetonic extract of Carpesium macrocephalum. Their chemical structures were elucidated by IR, UV, HR-ESI-MS, NMR 1D and 2D experiments, and the absolute configuration of 1-3 was resolved according to the (1)H NMR and CD spectrographic features of 1,3-/2,4-linked SLDs. Furthermore, 1 was unambiguously confirmed by Cu-Kα X-ray crystallographic analysis. Additionally, compounds 1 and 2 were revealed with potent cytotoxicities against human colon cancer HCT116 cells with IC50 values of 2.27 and 3.30 μM, respectively.
从大花金挖耳的丙酮提取物中分离出三种新的倍半萜内酯二聚体（SLD），即金挖耳内酯E-G（1-3），以及两种已知的单体单元，异土木香内酯（4）和土木香内酯（5）。通过红外光谱（IR）、紫外光谱（UV）、高分辨电喷雾电离质谱（HR-ESI-MS）、一维和二维核磁共振（NMR）实验对它们的化学结构进行了阐明，并根据1,3-/2,4-连接的SLD的氢核磁共振（¹H NMR）和圆二色（CD）光谱特征确定了1-3的绝对构型。此外，通过铜Kα射线晶体学分析明确证实了化合物1的结构。此外，化合物1和2对人结肠癌HCT116细胞显示出较强的细胞毒性，其半数抑制浓度（IC50）值分别为2.27和3.30 μM。
