Sundararaman Aravind, Srinivasan Sathiyaraj, Lee Sang-Seob
Department of Life Science, Kyonggi University, San 94-6, Iui-dong, Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Department of Life Science, School of Natural Science, Kyonggi University, San 94-6, Iui-dong, Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Antonie Van Leeuwenhoek. 2016 May;109(5):697-704. doi: 10.1007/s10482-016-0670-0. Epub 2016 Mar 4.
Two novel Gram-stain negative, motile, non-spore forming, facultative aerobic and short rod shaped bacterial strains, designated U15(T) and U32, were isolated from soil obtained from Ukraine. The sequence similarity of the 16S rRNA gene between strains U15(T) and U32 was found to be 99.5 %. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that new bacteria belong to the genus Noviherbaspirillum. The closest member of the genus was found to be Noviherbaspirillum malthae (97.0 %) followed by Noviherbaspirillum suwonensis (96.3 %). The novel isolates was observed to grow optimally at 30 °C and pH 7.0. The major fatty acids present in the two strains were identified as summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:0, and summed feature 8 (C18:1 ω7c/C18:1 ω6c). Ubiquinone 8 was identified as the respiratory quinone component for both the strains. The polar lipid (L) profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, unidentified aminolipid and unidentified Ls, and putrescine and 2-hydroxyputrescine as major polyamines. The G+C content of the DNA for the strain U15(T) was found to be 61.2 mol%. The DNA-DNA relatedness between U15(T) and U32 and closely related species was less than 40 %. Based on the polyphasic taxonomic analysis, a new species, Noviherbaspirillum humi sp. nov., is proposed. The type strain is strain U15(T) = JCM 19873(T) = KEMB 7305-102(T).
从乌克兰土壤中分离出两株革兰氏染色阴性、具运动性、不形成芽孢、兼性需氧且呈短杆状的新型细菌菌株，分别命名为U15(T)和U32。菌株U15(T)和U32之间16S rRNA基因的序列相似性为99.5%。基于16S rRNA基因序列的系统发育分析证实，这些新细菌属于新草螺菌属。该属中亲缘关系最近的成员是麦芽新草螺菌（97.0%），其次是水原新草螺菌（96.3%）。观察到这些新分离菌株在30℃和pH 7.0条件下生长最佳。这两株菌株中主要的脂肪酸被鉴定为特征性总和3（C16:1 ω7c/C16:1 ω6c）、C16:0和特征性总和8（C18:1 ω7c/C18:1 ω6c）。泛醌8被鉴定为这两株菌株的呼吸醌成分。极性脂质（L）谱包含磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、氨基磷脂、未鉴定的氨基脂质和未鉴定的L，以及腐胺和2-羟基腐胺作为主要的多胺。菌株U15(T)的DNA的G+C含量为61.2 mol%。U15(T)与U32以及密切相关物种之间的DNA-DNA相关性小于40%。基于多相分类分析，提出了一个新物种，即土壤新草螺菌（Noviherbaspirillum humi sp. nov.）。模式菌株是U15(T) = JCM 19873(T) = KEMB 7305-102(T)。
