Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2017 May;67(5):1508-1515. doi: 10.1099/ijsem.0.001747. Epub 2017 May 15.
A straw-coloured, Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain K-1-15T, was isolated from reclaimed grassland soil from Biratnagar, Morang, Nepal. This strain was non-spore-forming, catalase-negative and oxidase-positive. It was able to grow at 10-45 °C, pH 6.5-9.5 and 0-1.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, K-1-15T formed a distinct lineage within the family Oxalobacteraceae and was most closely related to members of the genera Herbaspirillum(96.99-95.34 % sequence similarity), Noviherbaspirillum(96.72-95.45 % sequence similarity) and Paraherbaspirillum (95.85 % sequence similarity). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of K-1-15T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C10 : 0 3-OH, and iso-C16 : 0. The genomic DNA G+C content of this novel strain was 65.2 mol %. The DNA-DNA relatedness between K-1-15T and Herbaspirillum massiliense DSM 25712T and Noviherbaspirillum soli LMG 26149T were 18.3 and 13.7 % repectively. On the basis of the results of morphological, physiological, chemotaxonomic and phylogenetic analyses, K-1-15T represents a novel species of the genus Noviherbaspirillum in the family Oxalobacteraceae, for which the name Noviherbaspirillum agri sp. nov. is proposed. The type strain is K-1-15T (=KEMB 9005-422T=KACC 18909T=JCM 31463T). Based on new data obtained in this study, we also propose the reclassification of Herbaspirillum massiliense as Noviherbaspirillum massiliense comb. nov. (type strain JC206T=CSUR P159T=DSM 25712T).
一株稻草色、革兰氏阴性、需氧、能动、杆状细菌，被命名为 K-1-15T，从尼泊尔莫朗地区比拉德讷格尔的再生草地土壤中分离得到。该菌株不产芽孢，过氧化氢酶阴性，氧化酶阳性。它可以在 10-45°C、pH 值 6.5-9.5 和 0-1.5%（w/v）NaCl 浓度下生长。该菌株通过多相分类法进行了分类学鉴定。根据 16S rRNA 基因序列分析的结果，K-1-15T 在 Oxalobacteraceae 科内形成一个独特的谱系，与 Herbaspirillum（96.99-95.34%序列相似性）、Noviherbaspirillum（96.72-95.45%序列相似性）和 Paraherbaspirillum（95.85%序列相似性）属的成员最为密切相关。唯一的呼吸醌是泛醌-8。极性脂分析表明存在磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。K-1-15T 的主要脂肪酸是总和特征 3（C16：1ω7c 和/或 C16：1ω6c）、C16：0、总和特征 8（C18：1ω7c 和/或 C18：1ω6c）、C10：0 3-OH 和 iso-C16：0。该新型菌株的基因组 DNA G+C 含量为 65.2 mol%。K-1-15T 与 Herbaspirillum massiliense DSM 25712T 和 Noviherbaspirillum soli LMG 26149T 的 DNA-DNA 相关性分别为 18.3%和 13.7%。基于形态、生理、化学生态和系统发育分析的结果，K-1-15T 代表 Oxalobacteraceae 科 Noviherbaspirillum 属的一个新种，建议将其命名为 Noviherbaspirillum agri sp. nov。模式菌株为 K-1-15T（=KEMB 9005-422T=KACC 18909T=JCM 31463T）。基于本研究获得的新数据，我们还建议将 Herbaspirillum massiliense 重新分类为 Noviherbaspirillum massiliense comb. nov.（模式菌株 JC206T=CSUR P159T=DSM 25712T）。
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