Dos Santos Neves Juliana, Franchin Marcelo, Rosalen Pedro Luiz, Omar Nadia Fayez, Dos Santos Mariana Albuquerque, Paschoal Jonas Augusto Rizzato, Novaes Pedro Duarte
Department of Morphology, Piracicaba Dental School, State University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
J Ethnopharmacol. 2016 May 13;183:151-158. doi: 10.1016/j.jep.2016.02.041. Epub 2016 Mar 2.
Hancornia speciosa Gomes, commonly known as Mangabeira, is a Brazilian native fruit tree belonging to the Apocynaceae family. In folk medicine, the latex obtained from Mangabeira's trunk has been used as an adjunct therapy for bone fractures. Few pharmacological studies on the Hancornia speciosa latex have been developed and despite its popular use for bone healing there is no data about its biological effect on bone.
The present study aimed to investigate the osteogenic potential of Hancornia speciosa latex in rat calvaria, as well as its phytochemical profile.
A neutral gel composition containing 5% latex was topical applied to a critical size bone defect and over intact calvaria of rats. Areas of newly formed bone on the borders of the defect and of calvaria periosteum were quantified, as well as the percentage of BrdU-positive cells and total cells in the periosteum at different periods of time after latex application. The cytotoxicity of the latex aqueous phase was evaluated in rat calvarial cells in vitro by MTT assay and its phytochemical profile was investigated by ESI-MS/MS.
The area of newly formed bone on the borders of the calvaria defect was larger in rats that received latex at 15 and 30 days of healing. After 3 days of latex application over the intact calvaria, the periosteum area was increased and newly formed bone was observed after 5 and 11 days. There was also an increase in periosteum cell proliferation and population followed latex application on calvaria (p<0.05). The latex aqueous phase limited rat calvarial cell viability in vitro in concentrations larger than 0.6mg/mL. Chlorogenic acid and naringenin-7-O-glucoside were identified in the latex aqueous phase, along with catechin and procyanidin compounds.
There was a stimulus for periosteum cell proliferation and bone formation when Hancornia speciosa latex was topically applied on rat calvaria. In addition, chlorogenic acid and naringenin-7-O-glucoside present in Hancornia speciosa latex may contribute to its effects on bone formation.
汉考尼亚树（Hancornia speciosa Gomes），俗称曼加贝拉树，是一种原产于巴西的果树，属于夹竹桃科。在民间医学中，从曼加贝拉树树干获取的乳胶已被用作骨折的辅助治疗方法。针对汉考尼亚树乳胶的药理学研究较少，尽管其在促进骨愈合方面被广泛使用，但尚无关于其对骨骼生物学作用的数据。
本研究旨在探究汉考尼亚树乳胶对大鼠颅骨的成骨潜力及其植物化学特征。
将含有5%乳胶的中性凝胶组合物局部应用于大鼠临界尺寸骨缺损处及完整颅骨上。对缺损边界和颅骨骨膜上新形成骨的面积进行量化，并在涂抹乳胶后的不同时间段，对骨膜中BrdU阳性细胞和总细胞的百分比进行量化。通过MTT法在体外评估乳胶水相在大鼠颅骨细胞中的细胞毒性，并通过电喷雾串联质谱（ESI-MS/MS）研究其植物化学特征。
在愈合15天和30天接受乳胶治疗的大鼠中，颅骨缺损边界上新形成骨的面积更大。在完整颅骨上涂抹乳胶3天后，骨膜面积增加，在5天和11天后观察到有新形成的骨。在颅骨上涂抹乳胶后，骨膜细胞增殖和数量也有所增加（p<0.05）。乳胶水相在浓度大于0.6mg/mL时会限制体外大鼠颅骨细胞的活力。在乳胶水相中鉴定出了绿原酸和柚皮素-7-O-葡萄糖苷，以及儿茶素和原花青素化合物。
当汉考尼亚树乳胶局部应用于大鼠颅骨时，对骨膜细胞增殖和骨形成有刺激作用。此外，汉考尼亚树乳胶中存在的绿原酸和柚皮素-7-O-葡萄糖苷可能有助于其对骨形成的作用。
