Laboratório de Farmacologia da Inflamação e do Óxido Nítrico, Programa de Desenvolvimento de Fármacos, ICB, Universidade Federal do Rio de Janeiro, Brazil.
J Ethnopharmacol. 2011 May 17;135(2):530-7. doi: 10.1016/j.jep.2011.03.059. Epub 2011 Apr 2.
Hancornia speciosa Gomes (Apocynaceae) is a tree that is widely distributed throughout Brazil. Its latex is collected and used extensively to treat acne, warts, diseases related to bursitis, and inflammation. In this work, we describe the anti-inflammatory effects of the latex.
The latex from Hancornia speciosa (0.06-1.3mg/kg, p.o.) and the reference drug acetylsalicylic acid (ASA, 200mg/kg, p.o.) were evaluated in analgesia (formalin-induced licking, acetic acid-induced contortions, and hot plate) and inflammation models (formalin-induced licking, paw oedema, and subcutaneous air pouch, with measurement of cell migration, exudate volume, protein extravasations, nitric oxide, prostaglandin E2, TNF-α, and IL-6, and expression of the enzymes inducible nitric oxide synthase and cyclooxygenase 2).
The latex from Hancornia speciosa significantly inhibited the number of writhings and the time that the animal spent licking the formalin-injected paw (second phase). Doses of 0.1-1.3mg/kg latex reduced carrageenan-induced rat paw oedema. However, only the highest doses (0.6 and/or 1.3mg/kg) reduced the oedema induced by bradykinin, histamine, and serotonin. The latex also inhibited inflammation induced by subcutaneous carrageenan injection, cell migration, exudate volume, protein extravasations, increased levels of inflammatory mediators (nitric oxide, prostaglandin E2, TNF-α, and IL-6) produced in the pouch, and increased expression of the enzymes nitric oxide synthase and cyclooxygenase 2.
Our results indicate that the latex obtained from Hancornia speciosa demonstrates significant anti-inflammatory activity through the inhibition of nitric oxide, PGE2, and cytokine production, thus confirming the popular use of this plant as an anti-inflammatory agent.
原产于巴西的人心果（夹竹桃科）是一种广泛分布的树木。其乳胶被广泛采集并用于治疗痤疮、疣、滑囊炎和炎症相关疾病。本工作描述了人心果乳胶的抗炎作用。
采用福尔马林诱导舔舐、醋酸诱导扭曲和热板试验评估人心果乳胶（0.06-1.3mg/kg，po）和对照药物乙酰水杨酸（ASA，200mg/kg，po）的镇痛作用，采用福尔马林诱导舔舐、爪肿胀和皮下气囊模型，测量细胞迁移、渗出物体积、蛋白渗出、一氧化氮、前列腺素 E2、TNF-α和 IL-6 以及诱导型一氧化氮合酶和环氧化酶 2 的表达，评价人心果乳胶的抗炎作用。
人心果乳胶显著抑制扭体次数和动物舔舐福尔马林注射爪的时间（第二阶段）。0.1-1.3mg/kg 剂量的人心果乳胶可减少角叉菜胶诱导的大鼠爪肿胀。然而，只有最高剂量（0.6 和/或 1.3mg/kg）可减少缓激肽、组胺和 5-羟色胺诱导的肿胀。乳胶还抑制了皮下角叉菜胶注射引起的炎症、细胞迁移、渗出物体积、蛋白渗出、炎症介质（一氧化氮、前列腺素 E2、TNF-α和 IL-6）水平升高以及囊袋中一氧化氮合酶和环氧化酶 2 表达增加。
本研究结果表明，人心果乳胶通过抑制一氧化氮、PGE2 和细胞因子产生显示出显著的抗炎活性，从而证实了该植物作为抗炎剂的民间用途。
