Nantulya V M, Bajyana Songa E, Hamers R
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Trop Med Parasitol. 1989 Sep;40(3):263-6.
An antigen-detection enzyme immunoassay based on a T. brucei group-specific monoclonal antibody was used for the detection of circulating antigens in several animal species experimentally infected with T. evansi stocks from Sudan, Indonesia, Thailand and South America. Circulating antigens were detected as early as 6 days after infection, and they persisted throughout the observation period of up to 60 days postinfection. In an analysis of sera from naturally infected water buffaloes from Thailand, the test identified all the animals with positive parasitological findings, and 3 additional cases that had not been diagnosed by parasitological techniques. In an analysis of sera from pigs on a farm in Thailand suspected of a T. evansi outbreak, the assay detected "antigenaemia" in 66.7% of the animals, with antigen titres ranging from 1:2 to 1.512.
一种基于布氏锥虫属特异性单克隆抗体的抗原检测酶免疫测定法，用于检测实验感染来自苏丹、印度尼西亚、泰国和南美洲的伊氏锥虫毒株的几种动物物种中的循环抗原。感染后最早6天即可检测到循环抗原，并且在感染后长达60天的观察期内持续存在。在对来自泰国自然感染水牛的血清分析中，该检测方法鉴定出所有寄生虫学检查结果呈阳性的动物，以及另外3例未通过寄生虫学技术诊断出的病例。在对泰国一个疑似伊氏锥虫爆发的农场的猪血清分析中，该测定法在66.7%的动物中检测到“抗原血症”，抗原滴度范围为1:2至1:512。
