Egbe-Nwiyi T N, Antia R E
Department of Veterinary Pathology, University of Maiduguri, Borno State, Nigeria.
J Small Anim Pract. 1995 May;36(5):229-32. doi: 10.1111/j.1748-5827.1995.tb02904.x.
A monoclonal antibody against a plasma membrane antigen of Trypanosoma rhodesiense was used for the detection of T brucei group-specific circulating antigen in 24 adult local dogs experimentally infected with T brucei brucei strain 8/18. Ten of the dogs were splenectomised and the remainder non-splenectomised (intact). Five dogs each from the splenectomised and intact groups were inoculated intravenously with trypanosomes. The infected dogs developed trypanosomiasis between days 4 and 8 after infection. The circulating antigens were detected as early as six days after infection and remained high until two weeks after treatment, when the circulating antigen declined. The detection of the antigens showed the existence of infection unlike the antibody test. The treatment of the infected dogs with diminazene aceturate (Berenil; Hoechst) at a dose of 7.0 mg/kg on day 21 after infection cleared all the parasites but elevated the circulating antigen levels. The antigen capture enzyme-linked immunosorbent assay is a useful diagnostic tool for complementing parasitological diagnosis, for detecting infection in the field and for ascertaining the efficacy of trypanocidal drugs.
一种针对罗德西亚锥虫质膜抗原的单克隆抗体被用于检测24只经布氏锥虫8/18株实验感染的成年本地犬体内布氏锥虫属特异性循环抗原。其中10只犬进行了脾切除，其余犬未进行脾切除（完整）。从脾切除组和完整组中各选取5只犬静脉接种锥虫。感染犬在感染后第4至8天出现锥虫病。循环抗原最早在感染后6天被检测到，并且一直保持高水平，直到治疗后两周，此时循环抗原水平下降。与抗体检测不同，抗原检测显示存在感染。在感染后第21天，用剂量为7.0 mg/kg的乙酰氨基苯脒（拜耳；赫斯特）对感染犬进行治疗，清除了所有寄生虫，但提高了循环抗原水平。抗原捕获酶联免疫吸附测定是一种有用的诊断工具，可用于补充寄生虫学诊断、检测野外感染以及确定杀锥虫药物的疗效。
