Rae P F, Luckins A G
Ann Trop Med Parasitol. 1984 Dec;78(6):587-96. doi: 10.1080/00034983.1984.11811869.
Antisera raised against Trypanosoma evansi and T. congolense were used in a double antibody sandwich enzyme immunosorbent assay (ELISA) to detect trypanosomal antigens in sera from experimentally infected rabbits and goats. Assays quantitated using reference antigen preparations and the homologous antisera showed that antigens could be detected at protein concentrations of 1.5 microgram ml-1. The antisera also cross-reacted with soluble antigens prepared from T. brucei and T. vivax at similar protein concentrations. In rabbits infected with T. evansi circulating antigens were first detected in serum four to eight days after infection and increased in levels thereafter. However, seven days after treatment with the trypanocidal drug suramin antigens were no longer detectable. In rabbits infected with T. congolense by Glossina morsitans, antigens were detected in serum 14 days after infective fly bite and had disappeared ten days after treatment with homidium chloride. Trypanosomal antigens were also detected in sera from goats infected with T. brucei and T. vivax using the antiserum prepared against T. evansi. Antigens were detected seven days after infection and maximal values observed between ten and 40 days post-infection, thereafter values declined but remained higher than pre-infection levels for up to 130 days.
用针对伊氏锥虫和刚果锥虫制备的抗血清，在双抗体夹心酶联免疫吸附测定（ELISA）中检测实验感染兔和山羊血清中的锥虫抗原。使用参考抗原制剂和同源抗血清进行的测定表明，在蛋白质浓度为1.5微克/毫升时可以检测到抗原。这些抗血清在相似蛋白质浓度下也与布氏锥虫和活泼锥虫制备的可溶性抗原发生交叉反应。在感染伊氏锥虫的兔中，感染后4至8天首次在血清中检测到循环抗原，此后水平升高。然而，在用杀锥虫药物苏拉明治疗7天后，抗原不再可检测到。在被舌蝇叮咬感染刚果锥虫的兔中，感染性蝇叮咬后14天在血清中检测到抗原，在用氯化萘啶治疗10天后抗原消失。使用针对伊氏锥虫制备的抗血清，也在感染布氏锥虫和活泼锥虫的山羊血清中检测到锥虫抗原。感染后7天检测到抗原，感染后10至40天观察到最大值，此后值下降，但在长达130天内仍高于感染前水平。
