在培养的小鼠细胞中一个不稳定遗传基因的过表达。
Overexpression of an unstably inherited gene in cultured mouse cells.
作者信息
Degnen G E, Miller I L, Adelberg E A, Eisenstadt J M
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3956-9. doi: 10.1073/pnas.74.9.3956.
Abstract
The specific activity of hypoxanthine-guanine phosphoribosyltransferase (IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) is increased up to 58-fold in unstable gene transferents produced by the transfer of cell-free chromosomal material from one mouse L cell line to another; the specific activity of this enzyme returns to normal levels when the transferred gene becomes stabilized. This phenomenon, which is not observed in comparable heterospecific transfers, may be an effect of gene dosage (multiple copies of the transferred genetic fragment in the unstable gene transferents), or it may represent an escape of the unstably inherited gene from the normal regulatory mechanisms of the recipient cell.
摘要
次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶（IMP：焦磷酸磷酸核糖基转移酶，EC 2.4.2.8）的比活性在通过将无细胞染色体物质从一种小鼠L细胞系转移到另一种细胞系所产生的不稳定基因转移体中增加了58倍；当转移的基因稳定后，该酶的比活性恢复到正常水平。在类似的异种特异性转移中未观察到这种现象，这可能是基因剂量的影响（不稳定基因转移体中转移的遗传片段有多个拷贝），或者它可能代表不稳定遗传的基因从受体细胞的正常调节机制中逃逸。
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5
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