哺乳动物组织培养细胞耐药变体中的酶改变
Altered enzymes in drug-resistant variants of mammalian tissue culture cells.
作者信息
Sharp J D, Capecchi N E, Capecchi M R
出版信息
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9. doi: 10.1073/pnas.70.11.3145.
Abstract
Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in the enzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (2) enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variants can arise from structural gene mutations.
摘要
为了分离出含有结构基因突变的小鼠L细胞变体，对两种选择程序进行了比较。在所得到的克隆细胞系变体中，发现次黄嘌呤磷酸核糖基转移酶（EC 2.4.2.8）（1）存在两种类型的改变：（1）动力学常数改变的酶，导致体内和体外对8-氮杂鸟嘌呤具有抗性；（2）体外热敏感性改变的酶。这些结果支持了组织培养细胞变体可由结构基因突变产生的观点。
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