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哺乳动物组织培养细胞耐药变体中的酶改变

Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

作者信息

Sharp J D, Capecchi N E, Capecchi M R

出版信息

Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9. doi: 10.1073/pnas.70.11.3145.

DOI:10.1073/pnas.70.11.3145
PMID:4522295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427189/
Abstract

Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in the enzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (2) enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variants can arise from structural gene mutations.

摘要

为了分离出含有结构基因突变的小鼠L细胞变体，对两种选择程序进行了比较。在所得到的克隆细胞系变体中，发现次黄嘌呤磷酸核糖基转移酶（EC 2.4.2.8）（1）存在两种类型的改变：（1）动力学常数改变的酶，导致体内和体外对8-氮杂鸟嘌呤具有抗性；（2）体外热敏感性改变的酶。这些结果支持了组织培养细胞变体可由结构基因突变产生的观点。

相似文献

1
Altered enzymes in drug-resistant variants of mammalian tissue culture cells.哺乳动物组织培养细胞耐药变体中的酶改变
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9. doi: 10.1073/pnas.70.11.3145.
2
Complementation in hybrid cells derived from mutagen-induced mouse clones deficient in hypoxanthine-guanine phosphoribosyl-transferase activity.源自诱变诱导的次黄嘌呤-鸟嘌呤磷酸核糖基转移酶活性缺陷小鼠克隆的杂交细胞中的互补作用。
Exp Cell Res. 1974 Oct;88(2):410-4. doi: 10.1016/0014-4827(74)90261-4.
3
Cells with phosphoribosyl transferase activity recovered from mouse cells resistant to 8-azaguanine.从对8-氮杂鸟嘌呤具有抗性的小鼠细胞中分离得到具有磷酸核糖基转移酶活性的细胞。
Nat New Biol. 1973 Feb 14;241(111):194-6. doi: 10.1038/newbio241194a0.
4
The role of 8-azaguanine in the selection from human diploid cells of mutants deficient in hypoxanthine-guanine-phosphoribosyl-transferase (HGPRT).8-氮杂鸟嘌呤在从人二倍体细胞中筛选次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷型突变体中的作用。
Mutat Res. 1974 Jul;24(1):55-68. doi: 10.1016/0027-5107(74)90047-5.
5
Azaguanine resistant hamster cell lines not deficient in hypoxanthine-guanine phosphoribosyl transferase.对氮杂鸟嘌呤有抗性的仓鼠细胞系，次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶不缺乏。
J Cell Physiol. 1973 Feb;81(1):97-100. doi: 10.1002/jcp.1040810112.
6
Biochemical characteristics of 8-azaguanine resistant human lymphoblast mutants selected in vitro.
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Proc Natl Acad Sci U S A. 1973 Jul;70(7):1998-2002. doi: 10.1073/pnas.70.7.1998.
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Immunological characterization of hypoxanthine-guanine phosphoribosyl transferase mutants of mouse L cells: evidence for mutations at different loci in the HGPRT gene.小鼠L细胞次黄嘌呤-鸟嘌呤磷酸核糖转移酶突变体的免疫学特性：HGPRT基因不同位点发生突变的证据
J Cell Physiol. 1975 Apr;85(2 Pt 1):307-20. doi: 10.1002/jcp.1040850217.
10
Selective degradation of abnormal proteins in mammalian tissue culture cells.哺乳动物组织培养细胞中异常蛋白质的选择性降解。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4732-6. doi: 10.1073/pnas.71.12.4732.

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Cells. 2022 Jan 21;11(3):360. doi: 10.3390/cells11030360.
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Properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.携带单纯疱疹病毒2型胸苷激酶基因的细胞特性：回复为胸苷激酶阴性表型的机制
J Virol. 1980 Dec;36(3):746-55. doi: 10.1128/JVI.36.3.746-755.1980.
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Heterogeneity in the rate of benzo[a]pyrene metabolism in single cells: quantitation using flow cytometry.单细胞中苯并[a]芘代谢速率的异质性：使用流式细胞术进行定量分析。
Mol Cell Biol. 1982 Jun;2(6):625-32. doi: 10.1128/mcb.2.6.625-632.1982.
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The immunogenic properties of drug-resistant murine tumor cells do not correlate with expression of the MDR phenotype.耐药小鼠肿瘤细胞的免疫原性特性与多药耐药（MDR）表型的表达不相关。
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Induction of prolactin-deficient variants of GH3 rat pituitary tumor cells by ethyl methanesulfonate: reversion by 5-azacytidine, a DNA methylation inhibitor.甲磺酸乙酯诱导GH3大鼠垂体瘤细胞催乳素缺陷变体：DNA甲基化抑制剂5-氮杂胞苷的回复作用
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本文引用的文献

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THE INOSINIC ACID PYROPHOSPHORYLASE ACTIVITY OF MOUSE FIBROBLASTS PARTIALLY RESISTANT TO 8-AZAGUANINE.对8-氮杂鸟嘌呤有部分抗性的小鼠成纤维细胞的肌苷酸焦磷酸化酶活性
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Evidence for intergenic complementation in hybrid cells derived from two human diploid strains each carrying an X-linked mutation.来自两个人类二倍体细胞株（每个细胞株都携带一个X连锁突变）的杂交细胞中基因间互补的证据。
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Selective and nonselective isolation of temperature-sensitive mutants of mouse L-cells and their characterization.
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Micro-scale peptide mapping method for identification of variant proteins.用于鉴定变异蛋白的微尺度肽图谱分析方法。
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Temperature sensitive conditional mutants of monkey kidney cells.
Nature. 1969 Sep 27;223(5213):1380-1. doi: 10.1038/2231380a0.