中国仓鼠卵巢细胞中分离中期染色体对共显性标记的转移
Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.
作者信息
Spandidos D A, Siminovitch L
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3480-4. doi: 10.1073/pnas.74.8.3480.
Abstract
Codominant mutations to methotrexate and ouabain resistance in Chinese hamster ovary cells can be transferred to recipient Chinese hamster ovary cells by isolated metaphase chromosomes. For methotrexate, both the structural change and the increased activity of dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase EC 1.5.1.3), characteristic of the donor cells, are observed in the transferents (cells that carry and express functions derived by chromosome transfer). The transferents are unstable in the absence of selection although stable clones can be isolated. From results obtained by fractionation of chromosomes and transfer to recipients, the methotrexate and ouabain markers can be assigned to the middle and large size-classes of chromosomes, respectively. By fractionation and transfer of chromosomes, from transferents to new recipients, evidence has been obtained that chromosome integration is not restricted to a particular chromosomal site in the recipient.
摘要
中国仓鼠卵巢细胞中对甲氨蝶呤和哇巴因耐药的共显性突变可通过分离的中期染色体转移至受体中国仓鼠卵巢细胞。对于甲氨蝶呤，在转移细胞（通过染色体转移携带并表达衍生功能的细胞）中可观察到供体细胞特有的结构变化以及二氢叶酸还原酶（5,6,7,8 - 四氢叶酸：NADP +氧化还原酶，EC 1.5.1.3）活性增加。在无选择条件下，转移细胞不稳定，不过可分离出稳定克隆。通过对染色体进行分级分离并转移至受体，甲氨蝶呤和哇巴因标记分别可定位到中等大小和大尺寸的染色体类别。通过对染色体进行分级分离并从转移细胞转移至新受体，已获得证据表明染色体整合并不局限于受体中的特定染色体位点。
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1
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Exp Cell Res. 1970 Mar;59(3):469-78. doi: 10.1016/0014-4827(70)90656-7.
2
Two types of ribosome in mouse-hamster hybrid cells.小鼠-仓鼠杂交细胞中的两种核糖体。
Nat New Biol. 1971 Mar 10;230(10):52-4. doi: 10.1038/newbio230052a0.
3
Isolation and biochemical characterization of folate deficient mutants of Chinese hamster cells.中国仓鼠细胞叶酸缺陷型突变体的分离及生化特性分析
Cell. 1974 Jul;2(3):173-82. doi: 10.1016/0092-8674(74)90091-9.
4
Transfer of genetic information by purified metaphase chromosomes.通过纯化的中期染色体进行遗传信息的转移。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1258-62. doi: 10.1073/pnas.70.4.1258.
5
Mammalian cells with altered forms of RNA polymerase II.具有改变形式的RNA聚合酶II的哺乳动物细胞。
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6
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Virology. 1974 Sep;61(1):303-5. doi: 10.1016/0042-6822(74)90266-9.
7
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8
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10
Alpha-amanitin resistance: a dominant mutation in CHO cells.
Cell. 1975 Feb;4(2):167-72. doi: 10.1016/0092-8674(75)90123-3.
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