Zhang Q, Zeng Q F, Cotter P, Applegate T J
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907 Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, Sichuan Province, 625014, PR China.
Poult Sci. 2016 Jun 1;95(6):1348-55. doi: 10.3382/ps/pew032. Epub 2016 Mar 4.
Two experiments were conducted to determine the dietary threonine (Thr) requirement of Pekin ducks from hatch to 14 d of age. In experiment 1, practical corn-soybean meal diets were formulated to contain 0.78, 0.84, 0.90, 0.96, and 1.02% Thr (0.74, 0.83, 0.88, 0.92, and 1.00% Thr on an analyzed basis). In experiment 2, corn-soybean meal diets supplemented with 11 crystalline amino acids were formulated to contain 0.60, 0.70, 0.80, 0.90, 1.00, and 1.10% Thr (0.60, 0.75, 0.89, 0.95, 1.01, and 1.09% Thr on an analyzed basis). In both experiments, diets were fed to 8 replicate cages with 6 male ducks per cage. Body weight and feed intake from each cage were recorded weekly. At 14 d of age, breast meat, ileal digesta, and serum were collected to determine breast meat yield, mucin secretion, and serology parameters. In both studies, the estimated Thr requirement (expressed as % dietary Thr basis) for 14 d BW and BW gain (BWG) by quadratic broken-line (QBL) regression were similar, which were 0.87 and 0.86%, respectively. Additional measures in both experiments resulted in Thr requirements via QBL regression in rank order of crude mucin secretion < breast meat yield < serum immune activity. Summing up the estimates from both studies, the Thr requirement ranged from a low of 0.81% to maximize feed intake (FI) to a high of 1.00% to maximize serum Rb L100 by QBL regression. Correspondingly, the Thr requirement varied between a low of 0.90% to maximize crude mucin secretion on a dry matter intake (DMI) basis and a high of 0.98% to maximize feed-to-gain when using quadratic regression.
进行了两项试验,以确定北京鸭从出壳至14日龄的日粮苏氨酸(Thr)需要量。在试验1中,配制的实用玉米-豆粕型日粮含苏氨酸0.78%、0.84%、0.90%、0.96%和1.02%(分析基础上分别为0.74%、0.83%、0.88%、0.92%和1.00%)。在试验2中,配制的添加11种晶体氨基酸的玉米-豆粕型日粮含苏氨酸0.60%、0.70%、0.80%、0.90%、1.00%和1.10%(分析基础上分别为0.60%、0.75%、0.89%、0.95%、1.01%和1.09%)。在两项试验中,日粮饲喂8个重复栏,每栏6只公鸭。每周记录每个栏的体重和采食量。在14日龄时,采集胸肉、回肠食糜和血清,以测定胸肉产量、黏蛋白分泌和血清学参数。在两项研究中,通过二次折线(QBL)回归得出的14日龄体重(BW)和体重增加(BWG)的估计苏氨酸需要量(以日粮苏氨酸百分比表示)相似,分别为0.87%和0.86%。两项试验中的其他测定结果显示,通过QBL回归得出的苏氨酸需要量按粗黏蛋白分泌<胸肉产量<血清免疫活性的顺序排列。综合两项研究的估计值,通过QBL回归得出的苏氨酸需要量范围为:低至0.81%以最大化采食量(FI),高至1.00%以最大化血清Rb L100。相应地,以干物质采食量(DMI)为基础,苏氨酸需要量在低至0.90%以最大化粗黏蛋白分泌和高至0.98%以最大化料重比之间变化(使用二次回归时)。