Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of, Ministry of Education, Ministry of Agriculture and Rural Affaires, Sichuan Province 611130, China.
Habio Bio-tech Co. Ltd., Mianyang, China, 610000.
Poult Sci. 2020 Jul;99(7):3557-3566. doi: 10.1016/j.psj.2020.03.047. Epub 2020 Apr 27.
This study aimed to investigate to the effects of dietary CP levels and protease supplementation on growth performance, carcass traits, meat quality, nutrients utilization, and standardized ileal digestibility of amino acid in Pekin ducks fed a complex diet. A total of 960 14-day-old male ducks were weighed and randomly allotted to a 2 × 5 factorial arrangement of 10 treatments with 6 replicate pens per treatment and 16 ducks per pen fed to 49 D of age. Experimental factors included five dietary CP levels ranging from 13.5 to 17.5% and with or without protease (200 mg/kg) supplementation. Between day 28 to 34, the digestible and metabolizable trials were performed. Significant CP × protease interactions (P < 0.05) on breast meat yield, DM, energy and nitrogen utilization, as well as standardized ileal digestibility values of 7 amino acids were observed. Regardless of protease supplementation, ducks fed 13.5, 14.5, and 15.5% CP had a poorer (P < 0.05) growth performance and breast meat yield than ducks fed with 16.5 and 17.5% CP. Ducks fed 13.5% CP had a positive effect (P < 0.05) on meat quality, dietary DM, energy and nitrogen utilization as well as standardized ileal digestibility of amino acids. Protease supplementation increased (P < 0.05) DM and phosphorus retention and decreased (P < 0.05) shear force of breast meat, regardless of CP level; when CP = 14.5%, protease significantly increased (P < 0.05) breast muscle yield. The optimal CP requirement without or with protease supplementation for BWG and FI were 17.02 or 16.53% and 16.64 or 16.75%, respectively, based on linear broken-line regression.
本研究旨在探讨饲粮 CP 水平和蛋白酶添加对复杂饲粮饲养的肉鸭生长性能、屠宰性能、肉品质、养分利用率和氨基酸回肠标准消化率的影响。选用 14 日龄雄性北京鸭 960 只,随机分为 2×5 因子设计的 10 个处理,每个处理设 6 个重复,每个重复 16 只鸭,试验期 49 d。试验因素包括 5 种 CP 水平(13.5%、14.5%、15.5%、16.5%和 17.5%)和添加或不添加蛋白酶(200 mg/kg)。在 28~34 日龄进行消化代谢试验。结果表明,CP 与蛋白酶存在显著互作(P<0.05),对胸肌率、DM、能量和氮利用率以及 7 种氨基酸的回肠标准消化率有显著影响。无论是否添加蛋白酶,饲粮 CP 水平为 13.5%、14.5%和 15.5%时,肉鸭的生长性能和胸肌率均显著低于 CP 水平为 16.5%和 17.5%的肉鸭(P<0.05)。饲粮 CP 水平为 13.5%时,肉鸭的肉品质、饲粮 DM、能量和氮利用率以及氨基酸回肠标准消化率有显著提高(P<0.05)。无论 CP 水平如何,添加蛋白酶均可提高 DM 和磷的沉积率,降低胸肉剪切力(P<0.05);当 CP 为 14.5%时,蛋白酶显著提高了胸肌率(P<0.05)。基于线性二次模型回归分析,不添加或添加蛋白酶时,肉鸭获得最佳 BWG 和 FI 所需要的饲粮 CP 水平分别为 17.02%或 16.53%和 16.64%或 16.75%。