Sadeghnezhad Ehsan, Sharifi Mohsen, Zare-Maivan Hassan
Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Planta. 2016 Jul;244(1):75-85. doi: 10.1007/s00425-016-2476-8. Epub 2016 Mar 5.
A metabolic profiling including calculation of energy cost of amino acids biosynthesis in cultured cells of Scrophularia striata showed that methyl jasmonate-inducible oxidative stress elicited secondary metabolites formation derived from phenylalanine and tyrosine and increased energy cost for these amino acids biosynthesis. Understanding of the metabolic pathways in cell culture of Scrophularia striata, an aromatic plant species, facilitates means of production of pharmaceutical metabolites under oxidative stress. In this study, we evaluated the effects of MeJA on the S. striata metabolic pathway and the responses to oxidative stress. Exposure to methyl jasmonate (MeJA) affects plant growth, effectively induces production of reactive oxygen species (ROS) and inserts oxidative stress at the cellular level which results in alteration of primary metabolites and production of phenylepropanoid compounds. Cells treated with MeJA indicated increase in the activities of three antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPx) as well as intracellular H2O2 and MDA contents compared with mock-treated cells. High performance liquid chromatography (HPLC)-based metabolome analysis revealed dynamic metabolic changes in oxidatively stressed S. striata cells, e.g., general phenylpropanoid pathway, phenylethanoid-glycosides, lignans, and increased energy cost of biosynthesis and accumulation of amino acids. Furthermore, principal component analysis (PCA)-derived score plots demonstrated that MeJA affects cellular metabolism in S. striata cells and significantly alters metabolite composition under MeJA-inducible oxidative stress. These observations suggest that MeJA-elicited cell suspension cultures of S. striata balanced the production of primary and secondary metabolites in coordination with ROS-scavenging system.
对玄参培养细胞中氨基酸生物合成能量成本的代谢谱分析表明，茉莉酸甲酯诱导的氧化应激引发了源自苯丙氨酸和酪氨酸的次生代谢产物形成，并增加了这些氨基酸生物合成的能量成本。了解芳香植物玄参细胞培养中的代谢途径，有助于在氧化应激下生产药用代谢产物。在本研究中，我们评估了茉莉酸甲酯（MeJA）对玄参代谢途径的影响以及对氧化应激的反应。暴露于茉莉酸甲酯（MeJA）会影响植物生长，有效诱导活性氧（ROS）的产生，并在细胞水平上引发氧化应激，从而导致初级代谢产物的改变和苯丙烷类化合物的产生。与 mock 处理的细胞相比，用 MeJA 处理的细胞显示三种抗氧化酶（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、愈创木酚过氧化物酶（GPx））的活性增加，以及细胞内 H2O2 和 MDA 含量增加。基于高效液相色谱（HPLC）的代谢组分析揭示了氧化应激的玄参细胞中的动态代谢变化，例如一般苯丙烷途径、苯乙醇苷、木脂素，以及生物合成能量成本增加和氨基酸积累。此外，主成分分析（PCA）得出的得分图表明，MeJA 影响玄参细胞中的细胞代谢，并在 MeJA 诱导的氧化应激下显著改变代谢物组成。这些观察结果表明，MeJA 诱导的玄参细胞悬浮培养物与 ROS 清除系统协调平衡了初级和次级代谢产物的产生。
