Sagharyan Mostafa, Sharifi Mohsen, Samari Elaheh, Karimi Farah
Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Center of Excellence in Medicinal Plant Metabolites, Tarbiat Modares University, Tehran, Iran.
Sci Rep. 2024 Dec 28;14(1):30738. doi: 10.1038/s41598-024-78715-6.
Podophyllotoxin (PTOX), produced by Linum album, is a monolignol that participates in plant defense strategies. Our previous study established that methyl jasmonate (MeJA) significantly stimulates PTOX production in L. album cells. However; the mechanisms by which MeJA regulates PTOX biosynthesis are uncovered. In the present study, we demonstrated that MeJA induces a time-dependent hydrogen peroxide (HO) and salicylic acid (SA) accumulation but reduces nitric oxide (NO) generation in L. album cells. PTOX biosynthetic genes such as PAL, CCR, CAD, and PLR were upregulated in response to MeJA exposure. Furthermore, the results of RT-qPCR revealed a positive correlation between the expression of PTOX biosynthetic genes and MeJA-induced upregulation of four miRNAs such as miR156, miR159, miR172, and miR396 at 12 h. Generally, this study revealed that MeJA mediates PTOX biosynthesis in L. album cells by inducing HO and SA formation, which can probably upregulate the expression level of some miRNAs and biosynthetic genes in a redox balance-dependent manner.
由亚麻籽产生的鬼臼毒素（PTOX）是一种参与植物防御策略的单木酚。我们之前的研究表明，茉莉酸甲酯（MeJA）能显著刺激亚麻籽细胞中PTOX的产生。然而，MeJA调节PTOX生物合成的机制尚未被揭示。在本研究中，我们证明MeJA诱导亚麻籽细胞中过氧化氢（HO）和水杨酸（SA）随时间积累，但会减少一氧化氮（NO）的生成。响应MeJA处理，PTOX生物合成基因如PAL、CCR、CAD和PLR被上调。此外，RT-qPCR结果显示，在12小时时，PTOX生物合成基因的表达与MeJA诱导的四种miRNA（如miR156、miR159、miR172和miR396）上调之间存在正相关。总体而言，本研究表明MeJA通过诱导HO和SA的形成来介导亚麻籽细胞中PTOX的生物合成，这可能以氧化还原平衡依赖的方式上调一些miRNA和生物合成基因的表达水平。
