Yang Ying, Yu Hui, Li Hua, Wang Anli
College of Life Sciences, South China Normal University, Guangzhou, Guangdong 510631, China; College of Life Science, Foshan University, Foshan, Guangdong 528231, China.
College of Life Science, Foshan University, Foshan, Guangdong 528231, China.
Fish Shellfish Immunol. 2016 Apr;51:329-336. doi: 10.1016/j.fsi.2016.02.035. Epub 2016 Mar 2.
Aeromonas hydrophila is the causative pathogen of intestinal hemorrhage which has caused great economic loss in grass carp aquaculture. In order to understand the immunological response of grass carp to infection by A. hydrophila, the transcriptomic profiles of the spleens from infected and non-infected grass carp groups were obtained using HiSeq™ 2500 (Illumina). An average of 63 million clean reads per library was obtained, and approximately 80% of these genes were successfully mapped to the reference genome. A total of 1591 up-regulated and 530 down-regulated genes were identified. Eight immune-related categories involving 105 differently expressed genes were scrutinized. 16 of the differently expressed genes involving immune response were further validated by qRT-PCR. Our results provide valuable information for further analysis of the mechanisms of grass carp defense against A. hydrophila invasion.
嗜水气单胞菌是导致肠道出血的病原体，已给草鱼养殖造成巨大经济损失。为了解草鱼对嗜水气单胞菌感染的免疫反应，使用HiSeq™ 2500（Illumina）获得了感染和未感染嗜水气单胞菌的草鱼组脾脏的转录组图谱。每个文库平均获得6300万个 clean reads，其中约80%的基因成功比对到参考基因组。共鉴定出1591个上调基因和530个下调基因。对涉及105个差异表达基因的8个免疫相关类别进行了仔细研究。通过qRT-PCR进一步验证了16个涉及免疫反应的差异表达基因。我们的结果为进一步分析草鱼抵御嗜水气单胞菌入侵的机制提供了有价值的信息。
