Yang Ying, Yu Hui, Li Hua, Wang Anli, Yu Hai-Yi
College of Life Science, Foshan University, Foshan, Guangdong 528231, China; College of Life Science, South China Normal University, Guangzhou, Guangdong 510631, China.
College of Life Science, Foshan University, Foshan, Guangdong 528231, China.
Fish Shellfish Immunol. 2016 Nov;58:89-95. doi: 10.1016/j.fsi.2016.09.014. Epub 2016 Sep 12.
Exposure to high temperature is an inherent feature of grass carp culture in southern China and juvenile grass carps are predisposed to infectious disease in this condition. To understand how high temperature impacts the immune response to pathogens in grass carp, the transcriptomic profiles of the spleens from immune injected grass carp groups undergoing heat stress and normal temperature were investigated. An average of 72 million clean reads per library was obtained, and approximate 80% of these genes were successfully mapped to the reference genome. A total of 2287 up-regulated and 1068 down-regulated genes were identified. 10 immune-related categories involving 90 differently expressed genes were scrutinized. Expression patterns of 18 differentially expressed genes involving in immune response were validated by quantitative real-time RT-PCR. These results provide further significant insights into the influence mechanism of high temperature to immune response in grass carp.
在中国南方，草鱼养殖的一个固有特点是会受到高温影响，在此条件下草鱼幼鱼易患传染病。为了解高温如何影响草鱼对病原体的免疫反应，研究了热应激和常温下免疫注射草鱼群体脾脏的转录组概况。每个文库平均获得7200万个clean reads，其中约80%的基因成功映射到参考基因组。共鉴定出2287个上调基因和1068个下调基因。对涉及90个差异表达基因的10个免疫相关类别进行了仔细研究。通过定量实时RT-PCR验证了18个参与免疫反应的差异表达基因的表达模式。这些结果为深入了解高温对草鱼免疫反应的影响机制提供了进一步的重要见解。
