Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 336-708, Republic of Korea.
Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA; Department of Biological Sciences, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, Troy, NY, USA.
Biotechnol Adv. 2016 Sep-Oct;34(5):634-662. doi: 10.1016/j.biotechadv.2016.02.012. Epub 2016 Mar 3.
In this review, we address recent advances made in pathway engineering, directed evolution, and systems/synthetic biology approaches employed in the production and modification of flavonoids from microbial cells. The review is divided into two major parts. In the first, various metabolic engineering and system/synthetic biology approaches used for production of flavonoids and derivatives are discussed broadly. All the manipulations/engineering accomplished on the microorganisms since 2000 are described in detail along with the biosynthetic pathway enzymes, their sources, structures of the compounds, and yield of each product. In the second part of the review, post-modifications of flavonoids by four major reactions, namely glycosylations, methylations, hydroxylations and prenylations using recombinant strains are described.
在这篇综述中，我们讨论了在微生物细胞中生产和修饰类黄酮的途径工程、定向进化和系统/合成生物学方法方面的最新进展。本综述分为两大部分。第一部分广泛讨论了用于生产类黄酮及其衍生物的各种代谢工程和系统/合成生物学方法。详细描述了自 2000 年以来在微生物上完成的所有操作/工程，包括生物合成途径酶、它们的来源、化合物的结构和每种产物的产量。在综述的第二部分，描述了使用重组菌株通过四种主要反应（即糖基化、甲基化、羟基化和异戊烯基化）对类黄酮进行的后修饰。
