Global Health and Tropical Medicine (GHTM), Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, NOVA University of Lisbon, Lisbon, Portugal.
Medical Faculty, Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden.
J Med Virol. 2016 Sep;88(9):1511-20. doi: 10.1002/jmv.24510. Epub 2016 Mar 11.
Angola is a sub-Saharan country in southern Africa highly affected by diarrhoeal disease with limited epidemiological data regarding etiologic agents. This study was performed during 2012-2013, prior to rotavirus vaccine introduction, with the objective to detect and characterize the rotavirus strains circulating in four provinces of the country: Huambo, Luanda, Zaire, and Cabinda. A high rotavirus detection rate (35%, 117/334) was observed. G1 was the most common G-genotype (83.6%), whereas P[8] (50.9%) followed by P[6] (38.8%) were the most common P-types. G1P[8] was identified as the predominant combination (50%), followed by the unusual G1P[6] (29.3%). Strains such G2P[4], G8P[6], G9P[6], and G12P[6] were also found in lower frequencies (5.2-1.7%). The P[6] strains did not cluster in the phylogenetic trees according to their geographic origin or even the corresponding G-genotype, suggesting a limited number of recent introductions and extensive reassortment events. Our results represent the first report on rotavirus genotype profiles in Angola, showing a wide circulation of the unusual genotype G1P[6], and underline the importance of RV surveillance after the vaccine introduction. J. Med. Virol. 88:1511-1520, 2016. © 2016 Wiley Periodicals, Inc.
安哥拉是非洲南部的一个撒哈拉以南国家，深受腹泻病的影响，但关于病原体的流行病学数据有限。本研究于 2012-2013 年进行，即在轮状病毒疫苗引入之前，目的是检测和分析该国四个省份（万博、罗安达、扎伊尔和卡宾达）流行的轮状病毒株。结果显示轮状病毒的检出率很高（35%，117/334）。G1 是最常见的 G 基因型（83.6%），而 P[8]（50.9%）和 P[6]（38.8%）是最常见的 P 型。鉴定出的主要组合是 G1P[8]（50%），其次是不常见的 G1P[6]（29.3%）。还发现了较低频率的 G2P[4]、G8P[6]、G9P[6]和 G12P[6]等株（5.2-1.7%）。P[6]株的聚类与其地理来源或相应的 G 基因型无关，提示近期引入和广泛重组事件的数量有限。我们的结果代表了安哥拉轮状病毒基因型谱的首次报告，显示了不常见基因型 G1P[6]的广泛流行，强调了疫苗引入后 RV 监测的重要性。J. Med. Virol. 88:1511-1520, 2016. © 2016 Wiley Periodicals, Inc.
