刚地弓形虫：一种包囊分子的鉴定与特性分析

Toxoplasma gondii: identification and characterization of a cyst molecule.

作者信息

Zhang Y W, Smith J E

机构信息

Department of Pure and Applied Biology, University of Leeds, United Kingdom.

出版信息

Exp Parasitol. 1995 Mar;80(2):228-33. doi: 10.1006/expr.1995.1028.

DOI:10.1006/expr.1995.1028

PMID:7534723

Abstract

Monoclonal antibodies were raised against the RRA strain of Toxoplasma gondii, and those with specific reactivity against tissue cysts were selected by differential FAST-ELISA screen. One clone, E7B2, reacted with a novel cyst-specific molecule of approximate molecular weight 29,000. The molecule is predominantly found in soluble fractions from intact cysts and bradyzoites, and immunofluorescence studies suggest that it accumulates in the matrix of the cyst. Western blotting experiments show the molecule to be a minor antigen recognized by polyclonal antisera from infected mice.

摘要

制备了针对刚地弓形虫RRA株的单克隆抗体，并通过差异快速酶联免疫吸附测定筛选出对组织包囊具有特异性反应的抗体。一个克隆E7B2与一种新的包囊特异性分子发生反应，该分子的分子量约为29,000。该分子主要存在于完整包囊和缓殖子的可溶性组分中，免疫荧光研究表明它积聚在包囊基质中。蛋白质印迹实验表明该分子是受感染小鼠的多克隆抗血清识别的次要抗原。