Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
Department of Basic Sciences and Environment, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Food Chem. 2016 Jul 15;203:16-22. doi: 10.1016/j.foodchem.2016.02.001. Epub 2016 Feb 2.
Crude chloroform, ethanol and acetone extracts of nineteen seaweed species were screened for their antioxidant and α-glucosidase inhibitory activity. Samples showing more than 60% α-glucosidase inhibitory activity, at a concentration of 1 mg/ml, were furthermore investigated using high-resolution α-glucosidase inhibition profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-HRMS-SPE-NMR). The results showed Ascophyllum nodosum and Fucus vesicolosus to be rich in antioxidants, equaling a Trolox equivalent antioxidant capacity of 135 and 108 mM Troloxmg(-1) extract, respectively. HR-bioassay/HPLC-HRMS-SPE-NMR showed the α-glucosidase inhibitory activity of A. nodosum, F. vesoculosus, Laminaria digitata, Laminaria japonica and Undaria pinnatifida to be caused by phlorotannins as well as fatty acids - with oleic acid, linoleic acid and eicosapentaenoic acid being the most potent with IC50 values of 0.069, 0.075 and 0.10 mM, respectively, and showing a mixed-type inhibition mode.
对 19 种海藻的粗氯仿、乙醇和丙酮提取物进行了抗氧化和α-葡萄糖苷酶抑制活性筛选。在 1mg/ml 浓度下，对显示出超过 60%α-葡萄糖苷酶抑制活性的样品进行了进一步研究，方法是使用高分辨率α-葡萄糖苷酶抑制谱结合高效液相色谱-高分辨率质谱-固相萃取-核磁共振波谱（HR 生物测定/HPLC-HRMS-SPE-NMR）。结果表明，裙带菜和泡叶藻富含抗氧化剂，其 Trolox 当量抗氧化能力分别为 135 和 108mM Troloxmg(-1)提取物。HR 生物测定/HPLC-HRMS-SPE-NMR 表明，裙带菜、泡叶藻、海带、裙带菜和羊栖菜的α-葡萄糖苷酶抑制活性是由岩藻黄质以及脂肪酸引起的，其中油酸、亚油酸和二十碳五烯酸的抑制活性最强，IC50 值分别为 0.069、0.075 和 0.10mM，表现出混合抑制模式。
