Department of Animal Food Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland.
Nutrients. 2023 Aug 11;15(16):3539. doi: 10.3390/nu15163539.
Diabetes mellitus is a serious metabolic disorder characterized by abnormal blood glucose levels in the body. The development of therapeutic strategies for restoring and maintaining blood glucose homeostasis is still in progress. Synthetic alpha-amylase and alpha-glucosidase inhibitors can improve blood glucose control in diabetic patients by effectively reducing the risk of postprandial hyperglycemia. Peptides of natural origin are promising compounds that can serve as alpha-glucosidase inhibitors in the treatment of type 2 diabetes. Potential alpha-glucosidase-inhibiting peptides obtained from aqueous and saline extracts from dry-cured pork loins inoculated with probiotic LAB were evaluated using in vitro and in silico methods. To identify the peptide sequences, liquid chromatography-mass spectrometry was used. For this purpose, in silico calculation methods were used, and the occurrence of bioactive fragments in the protein followed the ADMET approach. The most promising sequences were molecularly docked to test their interaction with the human alpha-glycosidase molecule (PDB ID: 5NN8). The docking studies proved that oligopeptides VATPPPPPPPK, DIPPPPM, TPPPPPPG, and TPPPPPPPK obtained by hydrolysis of proteins from ripening dry-cured pork loins showed the potential to bind to the human alpha-glucosidase molecule and may act effectively as a potential antidiabetic agent.
糖尿病是一种严重的代谢紊乱疾病，其特征是体内血糖水平异常。目前仍在研究恢复和维持血糖稳态的治疗策略。合成的α-淀粉酶和α-葡萄糖苷酶抑制剂可以通过有效降低餐后高血糖的风险来改善糖尿病患者的血糖控制。天然来源的肽是有前途的化合物，可以作为 2 型糖尿病治疗中的α-葡萄糖苷酶抑制剂。从接种益生菌 LAB 的干腌猪里脊肉的水提物和盐提物中获得的潜在α-葡萄糖苷酶抑制剂肽，使用体外和计算方法进行了评估。为了鉴定肽序列，使用了液相色谱-质谱法。为此，使用了计算方法，并根据 ADMET 方法对蛋白质中生物活性片段的出现进行了预测。最有前途的序列被分子对接以测试它们与人类α-糖苷酶分子（PDB ID：5NN8）的相互作用。对接研究证明，从成熟干腌猪里脊肉蛋白水解得到的寡肽 VATPPPPPPPK、DIPPPPM、TPPPPPPG 和 TPPPPPPPK 有潜力与人类α-葡萄糖苷酶分子结合，并可能有效地作为潜在的抗糖尿病药物发挥作用。
