Eldahshan Omayma A, Halim Ahmed F
Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Chem Biodivers. 2016 Jun;13(6):681-5. doi: 10.1002/cbdv.201500139. Epub 2016 May 24.
The essential oils isolated from the leaves and green branches of the Egyptian navel orange trees were analyzed by GC and GC/MS. A total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. The major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). The antimicrobial activities of both oils were evaluated using the agar-well diffusion method toward three representatives for each of Gram-positive bacteria, Gram-negative bacteria, and fungi. The oil of leaves was more effective as antimicrobial agent than that of the branches. Streptococcus pyogenes, Staphylococcus aureus, Salmonella typhimurium, and Aspergillus fumigatus were the most sensitive bacteria and fungi by the leaves oil.
采用气相色谱（GC）和气相色谱-质谱联用（GC/MS）对从埃及脐橙树叶和绿色枝条中分离得到的挥发油进行了分析。从树叶和树枝的挥发油中分别鉴定出33种和24种化合物，分别占总检测成分的96.0%和97.9%。主要成分是桧烯（36.5；33.0%）、萜品-4-醇（8.2；6.2%）、δ-3-蒈烯（7.0；9.4%）、柠檬烯（6.8；18.7%）、反式罗勒烯（6.7；6.1%）和β-月桂烯（4.5；4.4%）。采用琼脂孔扩散法对两种挥发油对革兰氏阳性菌、革兰氏阴性菌和真菌的三种代表菌的抗菌活性进行了评价。树叶挥发油作为抗菌剂比树枝挥发油更有效。化脓性链球菌、金黄色葡萄球菌、鼠伤寒沙门氏菌和烟曲霉是对树叶挥发油最敏感的细菌和真菌。
