Lubin Arnaud, Geerinckx Suzy, Bajic Steve, Cabooter Deirdre, Augustijns Patrick, Cuyckens Filip, Vreeken Rob J
Discovery Sciences, Janssen R&D, Beerse, Belgium.
Waters Corporation, Wilmslow, United Kingdom.
J Chromatogr A. 2016 Apr 1;1440:260-265. doi: 10.1016/j.chroma.2016.02.055. Epub 2016 Feb 22.
Eicosanoids, including prostaglandins and thromboxanes are lipid mediators synthetized from polyunsaturated fatty acids. They play an important role in cell signaling and are often reported as inflammatory markers. LC-MS/MS is the technique of choice for the analysis of these compounds, often in combination with advanced sample preparation techniques. Here we report a head to head comparison between an electrospray ionization source (ESI) and a new atmospheric pressure ionization source (UniSpray). The performance of both interfaces was evaluated in various matrices such as human plasma, pig colon and mouse colon. The UniSpray source shows an increase in method sensitivity up to a factor 5. Equivalent to better linearity and repeatability on various matrices as well as an increase in signal intensity were observed in comparison to ESI.
类二十烷酸，包括前列腺素和血栓烷，是由多不饱和脂肪酸合成的脂质介质。它们在细胞信号传导中起重要作用，常被报道为炎症标志物。液相色谱-串联质谱（LC-MS/MS）是分析这些化合物的首选技术，通常与先进的样品制备技术结合使用。在此，我们报告了电喷雾电离源（ESI）与新型大气压电离源（UniSpray）之间的直接比较。在人血浆、猪结肠和小鼠结肠等各种基质中评估了两种接口的性能。与ESI相比，UniSpray源的方法灵敏度提高了5倍。在各种基质上观察到了更好的线性和重复性，以及信号强度的增加。
