Huang Yajing, Wu Yanming, Chang Xinwen, Li Yan, Wang Kai, Duan Tao
Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, China.
Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, China.
Stem Cells Int. 2016;2016:9156731. doi: 10.1155/2016/9156731. Epub 2016 Jan 5.
Trophoblast cell dysfunction is involved in many disorders during pregnancy such as preeclampsia and intrauterine growth restriction. Few treatments exist, however, that target improving trophoblast cell function. Human umbilical cord mesenchymal stem cells (hUCMSCs) are capable of self-renewing, can undergo multilineage differentiation, and have homing abilities; in addition, they have immunomodulatory effects and paracrine properties and thus are a prospective source for cell therapy. To identify whether hUCMSCs can regulate trophoblast cell functions, we treated trophoblast cells with hUCMSC supernatant or cocultured them with hUCMSCs. Both treatments remarkably enhanced the migration and invasion abilities of trophoblast cells and upregulated their proliferation ability. At a certain concentration, hUCMSCs also modulated hCG, PIGF, and sEndoglin levels in the trophoblast culture medium. Thus, hUCMSCs have a positive effect on trophoblast cellular functions, which may provide a new avenue for treatment of placenta-related diseases during pregnancy.
滋养层细胞功能障碍与孕期的许多疾病有关，如先兆子痫和胎儿生长受限。然而，针对改善滋养层细胞功能的治疗方法很少。人脐带间充质干细胞（hUCMSCs）能够自我更新，可进行多谱系分化，并且具有归巢能力；此外，它们具有免疫调节作用和旁分泌特性，因此是细胞治疗的一个潜在来源。为了确定hUCMSCs是否能调节滋养层细胞功能，我们用hUCMSC上清液处理滋养层细胞或将它们与hUCMSCs共培养。两种处理均显著增强了滋养层细胞的迁移和侵袭能力，并上调了它们的增殖能力。在一定浓度下，hUCMSCs还调节了滋养层细胞培养基中的hCG、PIGF和可溶性内皮糖蛋白水平。因此，hUCMSCs对滋养层细胞功能有积极作用，这可能为孕期胎盘相关疾病的治疗提供一条新途径。
