用于巴西大西洋森林濒危物种格拉齐奥氏斯瓦茨木（豆科）的DNA微卫星标记。
DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest.
作者信息
Spoladore Janaína, Mansano Vidal F, Dias de Freitas Luan C, Sebbenn Alexandre M, Lemes Maristerra R
机构信息
Laboratório de Biologia Molecular de Plantas, Diretoria de Pesquisas, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil; Programa de Pós Graduação em Botânica Tropical, Escola Nacional de Botânica, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratório de Biologia Molecular de Plantas, Diretoria de Pesquisas, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
Appl Plant Sci. 2016 Feb 11;4(2). doi: 10.3732/apps.1500081. eCollection 2016 Feb.
PREMISE OF THE STUDY
Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics.
METHODS AND RESULTS
Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424-0.894). Cross-species amplifications of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. flaemingii, S. langsdorffii, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis).
CONCLUSIONS
The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species.
研究目的
为天然稀有且受威胁的树种巴西苏木（豆科）开发并鉴定一组DNA微卫星标记，以研究其保护遗传学。
方法与结果
通过富集基因组文库方法，为该物种分离并鉴定了10个DNA微卫星位点。平均期望杂合度为0.776（0.424 - 0.894）。对6个同属类群（无瓣巴西苏木无瓣变种、弗氏巴西苏木、朗氏巴西苏木、大穗巴西苏木、雅致巴西苏木桃金娘叶变种和大陆巴西苏木单型变种）成功测试了这些位点的跨物种扩增。
结论
开发的10个多态性微卫星标记信息丰富，将为研究巴西苏木及其他巴西苏木属物种的种群和保护遗传学提供宝贵资源。
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