Jun Yong-Woo, Park Heeju, Lee You-Kyung, Kaang Bong-Kiun, Lee Jin-A, Jang Deok-Jin
Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, Korea.
Department of Applied Biology, College of Ecology and Environment, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, Korea.
FEBS Lett. 2016 Apr;590(7):954-61. doi: 10.1002/1873-3468.12123. Epub 2016 Mar 28.
Dual A-kinase anchoring protein 1a (D-AKAP1a, AKAP1) regulates cAMP signaling in mitochondria. However, it is not clear how D-AKAP1a is associated with mitochondria. In this study, we show that D-AKAP1a is a transmembrane protein in the mitochondrial outer membrane (MOM). We revealed that the N-terminus of D-AKAP1a is exposed to the intermembrane space of mitochondria and that its C-terminus is located on the cytoplasmic side of the MOM. Moderate hydrophobicity and the positively charged flanking residues of the transmembrane domain of D-AKAP1a were important for targeting. Taken together, D-AKAP1a can be classified as a signal-anchored protein in the MOM. Our topological study provides valuable information about the molecular and cellular mechanisms of mitochondrial targeting of AKAP1.
双A激酶锚定蛋白1a（D-AKAP1a，AKAP1）调节线粒体中的cAMP信号传导。然而，尚不清楚D-AKAP1a如何与线粒体相关联。在本研究中，我们表明D-AKAP1a是线粒体外膜（MOM）中的一种跨膜蛋白。我们发现D-AKAP1a的N端暴露于线粒体的膜间隙，其C端位于MOM的细胞质侧。D-AKAP1a跨膜结构域的适度疏水性和带正电荷的侧翼残基对靶向定位很重要。综上所述，D-AKAP1a可归类为MOM中的信号锚定蛋白。我们的拓扑学研究为AKAP1线粒体靶向的分子和细胞机制提供了有价值的信息。
