Liu S, Liu C, Wang Z, Huang J, Zeng Q
Southern Medical University Department of Pediatrics, Zhujiang Hospital Guangzhou China.
Southern Medical University Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital Guangzhou China.
Cell Mol Biol (Noisy-le-grand). 2016 Feb 4;62(2):31-7.
Sepsis is a significant cause of morbidity and mortality worldwide. Acute respiratory distress syndrome (ARDS) is the most common and serious complication of sepsis, which presents with rapid and progressive acute onset respiratory failure. The microRNA-23a-5p, as a kind of circulating microRNA (miRNA), is considered to be a candidate biomarker for cardiovascular diseases. However, correlation between ARDS and miR-23a-5p is also elusive. This study aims to investigate the role of miR-23a-5p as the biomarkers for ARDS. In this study, ARDS was induced by intraperitoneally injected with LPS of Sprague-Dawley rats and serum and lung tissues were collected. The NR8383 macrophages were stimulated with LPS. TNF-α, IL-1β, and miR-23a-5p levels in serum, lung tissues and NR8383 were determined using SYBR-based miRNA quantitative real-time polymerase chain reactions (qRT-PCRs). The results indicated that serum miR-23a-5p was increased by 7 fold, 4 fold and 2 fold at 3 h, 6h, and 12h after injection of LPS, respectively. While the miR-23a-5p in NR8383 was elevated by 3.5 fold, 3 fold, 2.5 fold and 5 fold, at 3 h, 6h, 12h and 24h after stimulated with LPS, respectively. In conclusion, the miR-23a-5p might be employed as the potential biomarkers for ARDS in early stage.
脓毒症是全球发病和死亡的重要原因。急性呼吸窘迫综合征（ARDS）是脓毒症最常见且最严重的并发症，表现为快速进展的急性呼吸衰竭。微小RNA-23a-5p作为一种循环微小RNA（miRNA），被认为是心血管疾病的候选生物标志物。然而，ARDS与miR-23a-5p之间的相关性也尚不明确。本研究旨在探讨miR-23a-5p作为ARDS生物标志物的作用。在本研究中，通过腹腔注射脂多糖诱导Sprague-Dawley大鼠发生ARDS，并收集血清和肺组织。用脂多糖刺激NR8383巨噬细胞。采用基于SYBR的miRNA定量实时聚合酶链反应（qRT-PCR）测定血清、肺组织和NR8383中的肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和miR-23a-5p水平。结果表明，注射脂多糖后3小时、6小时和12小时，血清miR-23a-5p分别升高7倍、4倍和2倍。而在用脂多糖刺激后3小时、6小时、12小时和24小时，NR8383中的miR-23a-5p分别升高3.5倍、3倍、2.5倍和5倍。总之，miR-23a-5p可能作为ARDS早期的潜在生物标志物。
