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禽源大肠杆菌的血凝性、疏水性、产肠毒素性及耐药特性

Hemagglutination, hydrophobicity, enterotoxigenicity, and drug-resistance characteristics of avian Escherichia coli.

作者信息

Erganiş O, Kaya O, Corlu M, Istanbulluoğlu E

机构信息

Department of Bacteriology and Immunology, Faculty of Veterinary Medicine, Selçuk University, Konya, Turkey.

出版信息

Avian Dis. 1989 Oct-Dec;33(4):631-5.

PMID:2695047
Abstract

A total of 35 Escherichia coli isolates obtained from necropsy materials of hens with septicemia in the Konya region of Turkey were examined for hemagglutination (HA), cell-surface hydrophobicity, enterotoxigenicity, and drug resistance. HA tests were performed on live cultures with human (group A), bovine, avian (chicken), and guinea pig erythrocytes with and without mannose. Nine HA patterns were observed. Of the 35 isolates, 62.8% exhibited mannose sensitive hemagglutination (MSHA), 8.6% exhibited mannose resistant hemagglutination (MRHA), and 28.6% did not hemagglutinate. Of the isolates, 85.7% were hydrophobic by a salt aggregation test (SAT). Only three isolates were enterotoxigenic by a suckling mouse assay. The majority of the isolates were resistant to chloramphenicol, tetracycline, streptomycin, ampicillin, erythromycin, and trimethoprim + sulfamethoxazole but were highly sensitive to gentamicin and nalidixic acid.

摘要

对从土耳其科尼亚地区患有败血症的母鸡尸检材料中分离出的35株大肠杆菌进行了血凝(HA)、细胞表面疏水性、产肠毒素性和耐药性检测。用含和不含甘露糖的人(A组)、牛、禽(鸡)和豚鼠红细胞对活菌培养物进行HA试验。观察到9种HA模式。在35株分离株中,62.8%表现出甘露糖敏感血凝(MSHA),8.6%表现出甘露糖抗性血凝(MRHA),28.6%不发生血凝。通过盐聚集试验(SAT),85.7%的分离株具有疏水性。通过乳鼠试验,只有三株分离株产肠毒素。大多数分离株对氯霉素、四环素、链霉素、氨苄西林、红霉素和甲氧苄啶+磺胺甲恶唑耐药,但对庆大霉素和萘啶酸高度敏感。

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引用本文的文献

1
Characterization of Escherichia coli isolated from cases of avian colibacillosis.从禽大肠杆菌病病例中分离出的大肠杆菌的特性分析。
Can J Vet Res. 1993 Jul;57(3):146-51.
2
Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain.禽致病性大肠杆菌菌株血凝相关基因的分离与鉴定
Infect Immun. 1994 Apr;62(4):1369-80. doi: 10.1128/iai.62.4.1369-1380.1994.
3
A carAB mutant of avian pathogenic Escherichia coli serogroup O2 is attenuated and effective as a live oral vaccine against colibacillosis in turkeys.
禽致病性大肠杆菌O2血清型的carAB突变体减毒,作为活口服疫苗对火鸡大肠杆菌病有效。
Infect Immun. 1994 Sep;62(9):3766-72. doi: 10.1128/iai.62.9.3766-3772.1994.
4
Role of crl in avian pathogenic Escherichia coli: a knockout mutation of crl does not affect hemagglutination activity, fibronectin binding, or Curli production.Crl在禽致病性大肠杆菌中的作用:crl的敲除突变不影响血凝活性、纤连蛋白结合或卷曲菌毛的产生。
Infect Immun. 1992 Nov;60(11):4460-7. doi: 10.1128/iai.60.11.4460-4467.1992.