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在大肠杆菌中,相对表面疏水性、K1抗原和血凝活性之间存在关联。

Relative surface hydrophobicity, antigen K1 and haemagglutinating activity are associated in Escherichia coli.

作者信息

Herpay M, Czirók E, Szöllösy E, Fekete J, Gadó I, Milch H

机构信息

National Institute of Hygiene, Budapest, Hungary.

出版信息

Acta Microbiol Hung. 1991;38(1):17-28.

PMID:1810106
Abstract

Hydrophobic property of 136 Escherichia coli strains was examined by salt aggregation test (SAT). Out of the tested strains 61 were SAT positive. The correlations among the surface properties characterized by SAT and other phenotypical properties, e.g. mannose resistant haemagglutinating activity (MRHA), mannose sensitive haemagglutinating activity (MSHA), presence of antigen K1 and adsorption to Al(OH)3 gel were examined. The results showed that (i) Possession of antigen K1 provides the bacterial cell a hydrophilic character and covers its relative surface hydrophobicity; (ii) Correlation exists between the relative hydrophobicity of the bacteria determined by SAT and their haemagglutinating activity. SAT values are also influenced by non haemagglutinating fimbriae and also by other non fimbrial structures; (iii) The hydrophilic surface characters are mainly expressed by the results of adsorption to Al(OH)3 gel and the hydrophobic characters rather by the SAT values.

摘要

通过盐聚集试验(SAT)检测了136株大肠杆菌的疏水性。在测试的菌株中,61株SAT呈阳性。研究了以SAT表征的表面特性与其他表型特性之间的相关性,例如甘露糖抗性血凝活性(MRHA)、甘露糖敏感性血凝活性(MSHA)、抗原K1的存在以及对氢氧化铝凝胶的吸附。结果表明:(i)拥有抗原K1使细菌细胞具有亲水性,并掩盖其相对表面疏水性;(ii)由SAT测定的细菌相对疏水性与其血凝活性之间存在相关性。SAT值也受非血凝菌毛以及其他非菌毛结构的影响;(iii)亲水性表面特性主要通过对氢氧化铝凝胶的吸附结果来体现,而疏水性特性则主要通过SAT值来体现。

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