Miller L K
Bioessays. 1989 Oct;11(4):91-5. doi: 10.1002/bies.950110404.
Baculovirus vectors have proven useful in producing high levels of biologically active eukaryotic proteins and providing cellular fractions which are enriched in the protein of interest. Expression occurs in infected insect cells which also provide a suitable environment for post-translational modification and folding of the protein product. Stable baculovirus vectors can be constructed rapidly with a minimum of viral manipulation.
杆状病毒载体已被证明可用于大量生产具有生物活性的真核蛋白质,并提供富含目标蛋白质的细胞组分。表达发生在受感染的昆虫细胞中,这些细胞也为蛋白质产物的翻译后修饰和折叠提供了合适的环境。稳定的杆状病毒载体可以通过最少的病毒操作快速构建。