Heo Wan, Kim Eun Tae, Cho Sung Do, Kim Jun Ho, Kwon Seong Min, Jeong Ha Yeon, Ki Kwang Seok, Yoon Ho Baek, Ahn Young Dae, Lee Sung Sill, Kim Young Jun
National Institute of Animal Science, RDA, Cheonan 331-808, Korea .
Policy Research and Planning Team, Korea Institute for Advancement of Technology, Seoul 135-513, Korea .
Asian-Australas J Anim Sci. 2016 Mar;29(3):365-71. doi: 10.5713/ajas.15.0626. Epub 2016 Mar 1.
This study was aimed to evaluate the stability of conjugated linoleic acids (CLAs) by nano-encapsulation against in vitro ruminal biohydrogenation by microbial enzymatic conversion. CLAs (free fatty acid form of CLA [CLA-FFA], nano-encapsulated CLA-FFA, triglyceride form of CLA [CLA-TG], and nano-encapsulated CLA-TG) were used in the in vitro fermentation experiments. When Butyrivibrio fibrisolvens (B. fibrisolvens) was incubated with CLA-FFAs, the concentrations of cis-9, trans-11 CLA and vaccenic acid (VA) slightly was decreased and increased by nano-encapsulation, respectively. When B. fibrisolvens was incubated with CLA-TG, the concentrations of cis-9, trans-11 CLA and VA decreased, but these were increased when B. fibrisolvens was incubated with nano-encapsulated CLA-TG. The nano-encapsulation was more effective against the in vitro biohydrogenation activity of B.fibrisolvens incubated with CLA-FFA than with CLA-TG. In the in vitro ruminal incubation test, the total gas production and concentration of total volatile fatty acids incubated with nano-encapsulated CLA-FFA and CLA-TG were increased significantly after 24 h incubation (p<0.05). Nano-encapsulated CLA-FFA might, thus, improve the ruminal fermentation characteristics without adverse effects on the incubation process. In addition, nano-encapsulated CLA-FFA increased the population of Fibrobacter succinogenes and decreased the population of B. fibrisolvens population. These results indicate that nano-encapsulation could be applied to enhance CLA levels in ruminants by increasing the stability of CLA without causing adverse effects on ruminal fermentation.
本研究旨在评估通过纳米包封共轭亚油酸（CLA）来抵抗微生物酶促转化的体外瘤胃生物氢化作用的稳定性。CLA（游离脂肪酸形式的CLA [CLA-FFA]、纳米包封的CLA-FFA、甘油三酯形式的CLA [CLA-TG]和纳米包封的CLA-TG）用于体外发酵实验。当纤维丁酸弧菌（B. fibrisolvens）与CLA-FFA一起培养时，顺式-9，反式-11 CLA和vaccenic酸（VA）的浓度分别略有下降和通过纳米包封而增加。当B. fibrisolvens与CLA-TG一起培养时，顺式-9，反式-11 CLA和VA的浓度下降，但当B. fibrisolvens与纳米包封的CLA-TG一起培养时，这些浓度增加。纳米包封对与CLA-FFA一起培养的B.fibrisolvens的体外生物氢化活性比与CLA-TG一起培养时更有效。在体外瘤胃培养试验中，用纳米包封的CLA-FFA和CLA-TG培养24小时后，总产气量和总挥发性脂肪酸浓度显著增加（p<0.05）。因此，纳米包封的CLA-FFA可能改善瘤胃发酵特性而对培养过程没有不利影响。此外，纳米包封的CLA-FFA增加了琥珀酸纤维杆菌的数量并减少了B. fibrisolvens的数量。这些结果表明，纳米包封可用于通过提高CLA的稳定性来提高反刍动物体内的CLA水平，而不会对瘤胃发酵产生不利影响。
