Songsri Jiraporn, Aukkanimart Ratchadawan, Boonmars Thidarut, Ratanasuwan Panaratana, Laummaunwai Porntip, Sriraj Pranee, Sripan Panupan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Neglected Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Neglected Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand ; Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand ; Rajamangala University of Technology Isan Sakonnakhon Campus, Sakonnakhon 47160, Thailand.
Korean J Parasitol. 2016 Feb;54(1):47-53. doi: 10.3347/kjp.2016.54.1.47. Epub 2016 Feb 26.
Echinostomes are intestinal trematodes that infect a wide range of vertebrate hosts, including humans, in their adult stage and also parasitize numerous invertebrate and cold-blooded vertebrate hosts in their larval stages. The purpose of this study was to compare Echinostoma malayanum parasite growth, including worm recovery, body size of adult worms, eggs per worm, eggs per gram of feces, and pathological changes in the small intestine of experimental animals. In this study, 6-8-week-old male hamsters, rats, mice, and gerbils were infected with echinostome metacercariae and then sacrificed at day 60 post-infection. The small intestine and feces of each infected animal were collected and then processed for analysis. The results showed that worm recovery, eggs per worm, and eggs per gram of feces from all infected hamsters were higher compared with infected rats and mice. However, in infected gerbils, no parasites were observed in the small intestine, and there were no parasite eggs in the feces. The volume of eggs per gram of feces and eggs per worm were related to parasite size. The results of histopathological changes in the small intestine of infected groups showed abnormal villi and goblet cells, as evidenced by short villi and an increase in the number and size of goblet cells compared with the normal control group.
棘口吸虫是肠道吸虫，其成虫阶段可感染包括人类在内的多种脊椎动物宿主，幼虫阶段还寄生于众多无脊椎动物和冷血脊椎动物宿主。本研究的目的是比较马来棘口吸虫的寄生虫生长情况，包括虫体回收、成虫的体长、每条虫的产卵数、每克粪便中的虫卵数以及实验动物小肠的病理变化。在本研究中，6至8周龄的雄性仓鼠、大鼠、小鼠和沙鼠感染了棘口吸虫囊蚴，然后在感染后第60天处死。收集每只感染动物的小肠和粪便，然后进行分析处理。结果表明，与感染的大鼠和小鼠相比，所有感染仓鼠的虫体回收量、每条虫的产卵数和每克粪便中的虫卵数都更高。然而，在感染的沙鼠中，小肠未观察到寄生虫，粪便中也没有寄生虫卵。每克粪便中的虫卵量和每条虫的产卵数与寄生虫大小有关。感染组小肠组织病理学变化结果显示绒毛和杯状细胞异常，与正常对照组相比，绒毛短，杯状细胞数量和大小增加。
