从巴西南部海岸野生海洋物种粪便样本中分离出的肠球菌对抗菌剂的耐药性。
Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil.
作者信息
Prichula Janira, Pereira Rebeca Inhoque, Wachholz Guilherme Raffo, Cardoso Leonardo Almansa, Tolfo Neidimar Cezar Correa, Santestevan Naiara Aguiar, Medeiros Aline Weber, Tavares Maurício, Frazzon Jeverson, d'Azevedo Pedro Alves, Frazzon Ana Paula Guedes
机构信息
Microbiology, Immunology and Parasitology Department, Federal University of Rio Grande do Sul (UFRGS), Sarmento Leite 500, room 158, 90050-170, Porto Alegre, RS, Brazil; Gram-positive Coccus Laboratory, Federal University of Health Sciences of Porto Alegre (UFCSPA), Sarmento Leite 245, room 204, 90050-170, Porto Alegre, RS, Brazil.
Gram-positive Coccus Laboratory, Federal University of Health Sciences of Porto Alegre (UFCSPA), Sarmento Leite 245, room 204, 90050-170, Porto Alegre, RS, Brazil.
出版信息
Mar Pollut Bull. 2016 Apr 15;105(1):51-7. doi: 10.1016/j.marpolbul.2016.02.071. Epub 2016 Mar 5.
The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of Brazil. Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome.
本研究的目的是评估从巴西南部海岸发现的野生海洋物种（包括海鸟（n = 12）、海龟（n = 8）和哺乳动物（n = 3））的粪便样本中分离出的肠球菌的物种分布、抗菌药物耐药谱以及耐药基因的存在情况。根据表型和基因型特征对肠球菌进行分类，检测其对抗生素的敏感性，并通过聚合酶链反应检测tet(S)、tet(M)、tet(L)、mrsC和erm(B)基因的存在情况。粪肠球菌和屎肠球菌是最常见的物种。观察到单一（37.09%）、双重（25.80%）和多重（16.12%）抗生素耐药模式。对利福平的耐药最为常见。分别在60.15%、73.07%和23.07%的耐药菌株中检测到msrC、tet(M)和/或tet(L)基因。总之，这些物种中存在抗生素耐药菌株可能与食物网相互作用和水生污染物有关，或者与环境抗性组有关。
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