School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil.
CETESB - Environmental Company of São Paulo State, Av. Professor Frederico Hermann Jr, 345, São Paulo, SP 05459-900, Brazil.
J Water Health. 2024 Sep;22(9):1628-1640. doi: 10.2166/wh.2024.098. Epub 2024 Aug 23.
Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of , and . Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of , which is crucial to implement effective measures to preserve the integrity of coastal ecosystems.
由于人类活动，沿海水域的水质正面临越来越多的威胁。污水排放污染沿海水域，对环境和公众健康构成重大风险。我们旨在调查海滩水中是否存在抗生素耐药菌。在 10 个月的时间里，从巴西圣保罗州的四个海滩采集了样本。对 分离株进行基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/MS）和分子分析，以准确鉴定属和种。使用圆盘扩散法评估了 14 种抗生素的药敏性，然后进行多药耐药（MDR）分类。使用 PCR 扩增法检测了抗生素耐药基因（ARGs）。我们的研究结果显示了 、 和 的流行情况。在 130 株分离株中，有 118 株对多种抗生素具有耐药性。耐药基因的检测提供了抗生素耐药性在环境中转移的证据。我们的研究结果强调了需要不断进行研究和监测，以增强对 、 和 的致病性和抗菌耐药机制的了解，这对于实施有效措施保护沿海生态系统的完整性至关重要。
