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低频脉冲电磁场对髓核细胞中白细胞介素-1β和肿瘤坏死因子-α表达及分泌的影响。

Effect of a low-frequency pulsed electromagnetic field on expression and secretion of IL-1β and TNF-α in nucleus pulposus cells.

作者信息

Zou Jun, Chen Yufeng, Qian Jiale, Yang Huilin

机构信息

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

出版信息

J Int Med Res. 2017 Apr;45(2):462-470. doi: 10.1177/0300060516683077. Epub 2017 Feb 8.

DOI:10.1177/0300060516683077
PMID:28173722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5536647/
Abstract

Objective To investigate changes in nucleus pulposus cell expression and secretion of interleukin (IL)-1β and tumour necrosis factor (TNF)-α following stimulation with a low-frequency (LF) pulsed electromagnetic field (PEMF). Methods Primary rat nucleus pulposus cells were isolated and cultured in vitro, followed by stimulation with LF-PEMFs at a frequency of 2 Hz and different intensities, ranging from 0.5-3.0 A/m. Cells were observed for morphological changes, and proliferation rates were measured by cell viability counts. Expression of IL-1β and TNF-α within the nucleus pulposus cells was measured using western blotting, and levels of IL-1β and TNF-α secreted in the culture media were measured using enzyme-linked immunosorbent assay. Results Stimulation of nucleus pulposus cells with LF-PEMFs did not appear to affect cell morphology or nucleus pulposus cell IL-1β and TNF-α expression levels. LF-PEMFs did not significantly affect cell proliferation, however, levels of IL-1β and TNF-α secreted into the culture media were found to be significantly reduced in an intensity-dependent manner. Conclusion Low-frequency PEMF stimulation may inhibit secretion of IL-1β and TNF-α in cultured nucleus pulposus cells.

摘要

目的 探讨低频（LF）脉冲电磁场（PEMF）刺激后髓核细胞白细胞介素（IL）-1β和肿瘤坏死因子（TNF）-α的表达及分泌变化。方法 分离原代大鼠髓核细胞并进行体外培养，然后用频率为2 Hz、强度范围为0.5 - 3.0 A/m的低频脉冲电磁场进行刺激。观察细胞形态变化，通过细胞活力计数测量增殖率。使用蛋白质印迹法检测髓核细胞内IL-1β和TNF-α的表达，使用酶联免疫吸附测定法测量培养基中分泌的IL-1β和TNF-α水平。结果 低频脉冲电磁场刺激髓核细胞似乎不影响细胞形态或髓核细胞IL-1β和TNF-α的表达水平。低频脉冲电磁场对细胞增殖无显著影响，然而，发现培养基中分泌的IL-1β和TNF-α水平呈强度依赖性显著降低。结论 低频脉冲电磁场刺激可能抑制培养的髓核细胞中IL-1β和TNF-α的分泌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/21a5ffb2c02b/10.1177_0300060516683077-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/ac72abc1493c/10.1177_0300060516683077-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/d2636cfb5c68/10.1177_0300060516683077-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/b5e968995a66/10.1177_0300060516683077-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/21a5ffb2c02b/10.1177_0300060516683077-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/ac72abc1493c/10.1177_0300060516683077-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/d2636cfb5c68/10.1177_0300060516683077-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/b5e968995a66/10.1177_0300060516683077-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ea/5536647/21a5ffb2c02b/10.1177_0300060516683077-fig4.jpg

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[3]
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[4]
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[5]
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[6]
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[7]
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本文引用的文献

[1]
Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis.

Mol Med Rep. 2016-11

[2]
Tumor necrosis factor α-induced protein-3 protects zinc transporter 8 against proinflammatory cytokine-induced downregulation.

Exp Ther Med. 2016-9

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Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates.

J Vis Exp. 2016-5-9

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Int J Radiat Biol. 2016-5

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Disc in flames: Roles of TNF-α and IL-1β in intervertebral disc degeneration.

Eur Cell Mater. 2015-9-21

[10]
Mechanisms and therapeutic applications of electromagnetic therapy in Parkinson's disease.

Behav Brain Funct. 2015-9-7

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